Similar gene expression patterns characterize aging and oxidative stress in Drosophila melanogaster
Similar gene expression patterns characterize aging and oxidative stress in Drosophila melanogaster
复制标题
DOI：
10.1073/pnas.0307605101
复制
发表时间：
2004-05-18
影响因子：
11.1
通讯作者：
Tower, J
中科院分区：
文献类型：
--
作者：
Landis, GN;Abdueva, D;Tower, J
Affymetrix GeneChips were used to measure RNA abundance for approximate to13,500 Drosophila genes in young, old, and 100% oxygen-stressed flies. Data were analyzed by using a recently developed background correction algorithm and a robust multichip model-based statistical analysis that dramatically increased the ability to identify changes in gene expression. Aging and oxidative stress responses shared the up-regulation of purine biosynthesis, heat shock protein, antioxidant, and innate immune response genes. Results were confirmed by using Northerns and transgenic reporters. Immune response gene promoters linked to GFP allowed longitudinal assay of gene expression during aging in individual flies. Immune reporter expression in young flies was partially predictive of remaining life span, suggesting their potential as biomonitors of aging.
