Correlation of astroglial cell function on micro-patterned surfaces with specific geometric parameters
Correlation of astroglial cell function on micro-patterned surfaces with specific geometric parameters
复制标题
DOI：
10.1016/s0142-9612(99)00163-5
复制
发表时间：
1999-12-01
期刊：
影响因子：
14
通讯作者：
Bizios, R
中科院分区：
文献类型：
--
作者：
Kam, L;Shain, W;Bizios, R
Microcontact printing techniques were used to modify silicon substrates with arrays of hexagonal features of N-1[3-(trimethoxysilyl) propyl]diethylenetriamine (DETA) surrounded by octadecyltrichlorosilane (OTS), which are hydrophilic, cell-adhesive and hydrophobic, non-adhesive organosilanes, respectively. In the presence of serum proteins, LRM55 cell adhesion and morphology on these modified surfaces were best correlated to the width of the cell-adhesive features. On surfaces modified with small (5 mu m in width) cell-adhesive features, LRM55 cells elaborated only thin processes. As feature width was increased, cells on these surfaces exhibited increased cell spreading and elaborated wide processes. On surfaces modified with large (>35 mu m in width) features, single cells adhered to and spread upon individual DETA features. In a similar fashion, LRM55 cell adhesion density increased with increasing feature width; this correlation could be represented by a simple, second-order relation, and was independent of all other measures of pattern geometry. The results of this study provide evidence that micro-patterning may be effective in controlling astrocyte interaction with implant materials. (C) 1999 Elsevier Science Ltd. All rights reserved.
