Higher-level accumulation of foreign gene products in transgenic rice seeds by the callus-specific selection system
Higher-level accumulation of foreign gene products in transgenic rice seeds by the callus-specific selection system
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DOI：
10.1016/j.jbiosc.2008.09.002
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发表时间：
2009-01-01
影响因子：
2.8
通讯作者：
Takaiwa, Fumio
中科院分区：
文献类型：
--
作者：
Wakasa, Yuhya;Ozawa, Kenjirou;Takaiwa, Fumio
Transgenic rice plants that accumulate the hypocholesterolemic pentapeptide lactostatin (IIAEK) as a fusion protein with the endosperm seed storage protein glutelin were developed. Plants were selected from Agrobacterium-mediated transformants using a mutated-rice acetolactate synthase (mALS) marker protein expressed under the control of the rice callus-specific promoter (CSP) (CSP:mALS). Lactostatin accumulation levels were compared in mature seeds of 78 independent transgenic rice lines selected for either the CSP:mALS gene cassette or hygromycin phosphotransferase (HPT) under the control of the CaMV 35S promoter (35S:HPT). Transgenic rice seeds harboring a CSP:mALS gene cassette accumulated more (approximately 120-200% on average) lactostatin than those with a 35S:HPT gene cassette, and accumulation was largely independent of transgene copy number. Furthermore, transgenic rice seeds that were selected by HPT under the control of CSP (CSP:HPT) also accumulated more lactostatin (approximately 160% on average) than transgenic rice seeds with the 35S:HPT gene cassette. These results indicate that high-value bioactive peptides such as lactostatin can be produced at higher levels using callus-specific selection than by conventional constitutive selection. Specific expression of a marker gene at the selection stage may thus result in increased target peptide accumulation levels in seeds. (C) 2008, The Society for Biotechnology, Japan. All rights reserved.
