Microtubule dynamics in mitotic spindle displayed by polarized light microscopy
Microtubule dynamics in mitotic spindle displayed by polarized light microscopy
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DOI：
10.1091/mbc.9.7.1603
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发表时间：
1998-07-01
影响因子：
3.3
通讯作者：
Oldenbourg, R
中科院分区：
文献类型：
--
作者：
Inoué, S;Oldenbourg, R
第一个序列显示了来自非洲血百合Haemanthus katherinae的胚乳细胞，正在进行有丝分裂（图1）。AS Bajer和J. Molé-Bajer使用相差显微镜在琼脂和明胶层之间压平以提高可见度的细胞中观察到了该序列（Bajer和Mole-Bajer，1956，1986）。该序列生动地显示了染色体在中期平板上凝聚和排列时的情况（图1b）。与此同时，三个大的深色核仁（图1a）消失。然后染色体分裂并在后期分开（图1c）。最后，染色体在分裂末期被包装成两个子细胞核，从而变得解致密（图1d）。在细胞核之间，小的跳舞囊泡出现（图1c），排列并相互融合形成细胞板（图1d）。细胞板最终产生细胞壁，并将植物细胞一分为二。在下一个序列中，我们看到了一个复活节百合的花粉母细胞，百合，经历有丝分裂和细胞分裂（图2）。这些细胞同步进行两次分裂中的第一次，当花蕾正好22.4 mm长时形成四个花粉粒（图3）。收集该长度的芽，并在1800 g下离心3 min，以置换高度光散射的颗粒，并使细胞的其他内容物更加可见。在七分之八浓度的青蛙林格氏溶液中从离心的花蕾中切除花粉囊后，在存在补偿器的情况下在交叉偏振器之间观察细胞（图4）。用偏光显微镜以这种方式观察，细胞的区域
The first sequence shows an endosperm cell from the African blood lily, Haemanthus katherinae, undergoing mitosis (Figure 1). This sequence, captured by AS Bajer and J. Molé-Bajer using phase-contrast microscopy, was observed in cells that had been flattened between a layer of agar and gelatin to improve their visibility (Bajer and Mole-Bajer, 1956, 1986). The sequence vividly displays the chromosomes as they condense and align on the metaphase plate (Figure 1b). In the meantime the three large, dark nucleoli (Figure 1a) disappear. Then the chromosomes split and move apart in anaphase (Figure 1c). Finally the chromosomes become decondensed as they are packaged into two daughter nuclei in telophase (Figure 1d). Between the nuclei, small dancing vesicles appear (Figure 1c), align, and fuse with each other to form the cell plate (Figure 1d). The cell plate eventually gives rise to the cell walls and separates the plant cell into two. In the next sequence, we see the pollen mother cell of an Easter lily, Lilium longiflorum, undergoing mitosis and cell division (Figure 2). These cells synchronously undergo the first of their two divisions to form four pollen grains when the flower bud is exactly 22.4 mm long (Figure 3). A bud of this length was collected and centrifuged at 1800 g for 3 min to displace the highly light-scattering granules and to make the other contents of the cell more visible. After excising an anther from the centrifuged flower bud in seveneighths-strength frog Ringer’s solution, the cells were observed between crossed polarizers in the presence of a compensator (Figure 4). Observed with a polarizing microscope in this manner, regions of the cell