Cytogenetic variation among Cucumis accessions revealed by fluorescence in situ hybridization using ribosomal RNA genes as the probes
Cytogenetic variation among Cucumis accessions revealed by fluorescence in situ hybridization using ribosomal RNA genes as the probes
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DOI：
10.11352/scr.21.67
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发表时间：
2018
期刊：
影响因子：
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通讯作者：
A. Setiawan;C. Teo;S. Kikuchi;H. Sassa;Kenji Kato;T. Koba
中科院分区：
文献类型：
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作者：
A. Setiawan;C. Teo;S. Kikuchi;H. Sassa;Kenji Kato;T. Koba
瓜类梅洛是瓜类中最重要的园艺作物之一，果实大小和形状变异较大。虽然一些研究人员已经进行了比较核型在黄瓜属物种，详细的信息，分子细胞遗传学变异的关系，着丝粒和核糖体DNA（rDNA）基因座内的C。梅洛类群和其他甜瓜属物种仍然有限。本研究对C. metuliferus，C. hystrix和C.将梅洛分为梅洛亚种和土瓜属亚种。利用5S和45SrDNA探针进行荧光原位杂交（FISH），揭示了C.梅洛品种。C.梅洛亚种梅洛和稗草在5S和45SrDNA位点上具有高度的保守性。但45SrDNA信号强度和5SrDNA位置在不同的C.测试的梅洛物种。除P90外，其余10份材料的45SrDNA均为两对，且FISH信号强度相近。梅洛类群中变异较大，部分材料表现出与甜瓜亚类群相似的变异模式。agrestis的45SrDNA位点，而其它的45SrDNA位点有两对信号强度不同的位点。本研究结果为鉴别C.利用着丝粒DNA和rDNA探针对梅洛种质资源进行分析，这对甜瓜育种具有重要意义。
Cucumis melo, one of the most important horticultural crops in Cucumis genus, has great variation in fruit sizes and shapes. Although comparative karyotype in Cucumis species has been conducted by several researchers, detailed information on molecular cytogenetic variation in relation to centromeres and ribosomal DNA (rDNA) loci within C. melo groups and other Cucumis species are still limited. Here, we conducted physical mapping of centromeric DNA (Cmcent), and two rDNAs (5S and 45S) on C. metuliferus, C. hystrix, and nineteen accessions of C. melo that were classified into subspecies melo and agrestis. Fluorescence in situ hybridization (FISH) using 5S and 45S rDNA probes revealed cytogenetic variation among C. melo accessions. C. melo subsp. melo and agrestis showed high conservation in terms of the number of 5S and 45S rDNA loci. However, the 45S rDNA signal intensities and the 5S rDNA locations are variable among C. melo species tested. Most of the accessions in agrestis group had two pairs of 45S rDNA with similar FISH signal intensities, except P90. Great variations were observed in melo group where some of the accessions showed similar patterns to subsp. agrestis while others showed two pairs of 45S rDNA loci with variable signal intensities. Our findings provide useful information for distinguishing C. melo accessions using centromeric DNA and rDNA probes, which would be important for breeding programs in melon.