Connexin α1 and cell proliferation in the developing chick retina
Connexin α1 and cell proliferation in the developing chick retina
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DOI：
10.1006/exnr.1999.7027
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发表时间：
1999-04-01
影响因子：
5.3
通讯作者：
Mobbs, P
中科院分区：
文献类型：
--
作者：
Becker, DL;Mobbs, P
在眼睛的形成过程中，高水平的连接蛋白nl（连接蛋白43）在角膜、透镜和神经视网膜的组织内表达。为了确定连接蛋白arl是否在细胞增殖的调节中起作用，我们使用了一种新的反义技术来降低其在发育早期（胚胎第2-4天）的表达。将含有连接蛋白油的反义寡脱氧核苷酸（ODNs）的Pluronic凝胶应用于早期鸡胚的一只眼，导致α 1蛋白的快速和显著的减少，持续24-48小时。胚胎生长48小时后，ODN应用于一只眼睛，显示出显着减少的直径处理，相比，对侧未处理，眼睛。从接受治疗的眼睛上切下的切片显示，视网膜的尺寸也缩小了。TUNEL标记和碘化丙啶染色显示，在治疗和未治疗的眼睛的视网膜内的细胞凋亡是罕见的，因此，在视网膜面积的减少所带来的针对连接蛋白α 1的反义寡核苷酸不太可能是增加的细胞死亡的结果。然而，通过碘化丙啶染色显示的反义处理的视网膜的脑室区中的有丝分裂像的数量显著减少（P < 0.0001）至对侧未处理的对照眼中的53 +/-3.5%（n = 5）。其中一只眼睛假手术，用pluronic凝胶处理，或用正义ODN处理的胚胎在眼睛大小或神经视网膜内有丝分裂像的数量上没有显着变化。这些结果表明连接蛋白α 1介导的缝隙连接通讯在鸡视网膜神经发生的早期波控制中的作用。(C)北京：科学出版社.
During the formation of the eye, high levels of connexin nl (connexin 43) are expressed within the tissues of the cornea, lens, and neural retina. In order to determine whether connexin arl plays a role in the regulation of cell proliferation we have used a novel antisense technique to reduce its expression early in development (embryonic days 2-4). Application of Pluronic gel, containing antisense oligodeoxynucleotides (ODNs) to connexin oil, to one eye of early chick embryos results in a rapid and significant, reduction of alpha 1 protein which lasts for 24-48 h. Embryos grown for 48 h, after ODN application to one eye, showed a marked reduction in the diameter of the treated, compared to that of the contralateral untreated, eye. Sections cut from the treated eyes showed that the retina was also reduced in size. TUNEL labeling and staining with propidium iodide showed that apoptosis within the retinae of both treated and untreated eyes was rare and thus that the reduction in the area of the retina brought about by antisense ODNs directed at connexin alpha 1 was unlikely to be the result of increased cell, death. However, the number of mitotic figures in the ventricular zone of the antisense-treated retinae revealed by propidium iodide staining was significantly reduced (P < 0.0001) to 53 +/- 3.5% (n = 5) of that in the contralateral untreated control eyes. Embryos in which one eye was sham operated, treated with pluronic gel, or treated with sense ODN showed no significant changes in eye size or in the number of mitotic figures within the neural retina. These results point to a role for connexin alpha 1-mediated gap-junctional communication in controlling the early wave of neurogenesis in the chick retina. (C) 1999 Academic Press.