SPECIFIC SUPPRESSION OF RESPONSES TO LEISHMANIA-TROPICA BY A CLONED T-CELL LINE
SPECIFIC SUPPRESSION OF RESPONSES TO LEISHMANIA-TROPICA BY A CLONED T-CELL LINE
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DOI：
10.1038/305630a0
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发表时间：
1983-01-01
期刊：
影响因子：
64.8
通讯作者：
LIEW, FY
中科院分区：
文献类型：
--
作者：
LIEW, FY
大多数由热带利什曼原虫引起的皮肤利什曼病的临床病例主要包括自我愈合的病变，这些病变与通过延迟型超敏反应（DTH）检测到的强特异性细胞介导免疫（CMI）的发展有关1,2。另一个极端是易感个体，他们会发展成持续性或弥漫性的疾病。这种皮肤利什曼病的谱可以根据其遗传构成在近亲繁殖的小鼠品系中重现3。BALB/c小鼠特别易感：这种疾病可以用最小的感染剂量诱导，并且是不可阻挡的进展，最终以皮肤和致命的内脏转移而终止3 - 7。在这种情况下，潜在的治愈性CMI被特异性Lyt-1+2 -， I-J - T （Ts）细胞群的产生所消除8,9，其诱导可能继发于原发性巨噬细胞缺陷10,11，由单个主要的非h - 2连锁常染色体基因5,6控制。通过事先的亚致死（550 rad）照射可以预防t细胞的产生并治愈感染8。这种细胞的效力是这样的，只要106个细胞的过继转移就能完全逆转这种预防作用。鉴于它们的效力和与临床利什曼病的潜在相关性，我现在在克隆水平上研究t细胞，以进一步描述它们的功能特征和与效应机制的相互作用。我在这里报告了一种克隆的t细胞系，表达了对体外淋巴细胞增殖和体内诱导DTH toL的特异性抑制。tropicaantigens。
Most clinical cases of cutaneous leishmaniasis caused byLeishmania tropica majorconsist of self-healing lesions that are associated with the development of strong specific cell-mediated immunity (CMI) detected by delayed-type hypersensitivity (DTH)1,2. At the other extreme are susceptible individuals who develop persistent or diffuse forms of the disease. This spectrum of cutaneous leishmaniasis can be reproduced in in-bred mouse strains according to their genetic constitution3. BALB/c mice are exceptionally susceptible: the disease can be induced with minimum infecting doses and is inexorably progressive, terminating in cutaneous and fatal visceral metastasis3–7. In this case, potentially curative CMI is abrogated by the generation of a specific Lyt-1+2−, I-J−population of suppressor T (Ts) cells8,9, whose induction is probably secondary to a primary macrophage defect10,11controlled by a single major non-H–2-linked autosomal gene5,6. The generation of the Tscells can be prevented and infection cured by prior sublethal (550 rad) irradiation8. The potency of the Tscells is such that as few as 106cells transferred adoptively can completely reverse this prophylactic effect9. In view of their potency and potential relevance to clinical leishmaniasis, I have now studied Tscells at the clonal level to delineate further their functional characteristics and interaction with the effector mechanism. I report here a cloned T-cell line expressing specific suppression againstin vitrolymphocyte proliferation andin vivoinduction of DTH toL. tropicaantigens.