Investigating the impact of cholesterol on magnetically aligned sphingomyelin/cholesterol multilamellar vesicles using static 31P NMR
Investigating the impact of cholesterol on magnetically aligned sphingomyelin/cholesterol multilamellar vesicles using static 31P NMR
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DOI：
10.1016/j.chemphyslip.2010.04.001
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发表时间：
2010-06-01
影响因子：
3.4
通讯作者：
Alam, Todd M.
中科院分区：
文献类型：
--
作者：
Costello, Alison L.;Alam, Todd M.
用静态固体P-31 NMR光谱研究了胆固醇（5-40 mol%）对鞘磷脂/胆固醇多层囊泡（MLV）磁诱导取向的影响。假设囊泡的椭圆形变形，并作为胆固醇浓度和温度的函数进行监测的取向建模。此外，静态P-31化学位移各向异性（CSA）被用来评估发生在双层的运动和动力学变化的报告。探索的因素确定的磁取向的鞘磷脂/胆固醇双层从凝胶（S（O））的液晶（或液体有序，L（O））相的介绍和讨论。(C)2010爱思唯尔爱尔兰有限公司版权所有。
The effect of cholesterol (5-40 mol%) on the magnetic induced orientation of sphingomyelin/cholesterol multilamellar vesicles (MLVs) was examined using static solid state P-31 NMR spectroscopy. The orientation was modeled assuming an ellipsoidal deformation of the vesicles and was monitored as a function of cholesterol concentration and temperature. In addition, the static P-31 chemical shift anisotropy (CSA) was used to assess the motional and dynamical changes occurring in the bilayer are reported. An exploration of the factors determining the magnetic orientation in sphingomyelin/cholesterol bilayers from the gel (s(o)) to liquid crystalline (or liquid-ordered, l(o)) phases is presented and discussed. (C) 2010 Elsevier Ireland Ltd. All rights reserved.