Inhibition of nitrate reductase by azide in microalgae results in triglycerides accumulation
Inhibition of nitrate reductase by azide in microalgae results in triglycerides accumulation
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DOI：
10.1016/j.algal.2013.11.018
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发表时间：
2014
影响因子：
5.1
通讯作者：
T. Zalogin;U. Pick
中科院分区：
文献类型：
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作者：
T. Zalogin;U. Pick
叠氮化钠（Az）诱导甘油三酯（TAG）在绿色小球藻中积累，生长迟缓程度最小（Rachutin-Zalogin & Pick，联合手稿）。为了阐明这种作用的机制，呼吸，活性氧（ROS）的产生和硝酸还原酶（NR）的抑制的参与进行了研究。不同的呼吸抑制剂诱导轻微或没有TAG积累，排除呼吸作为主要Az目标。ROS发生器未能诱导大量TAG积累，但单线态氧猝灭剂DABCO抑制AZ诱导的TAG生物合成，并且这种抑制作用在30%D2O中被抑制。AZ诱导的TAG积累中观察到的硝酸盐，但不是在铵介质中，其中的增长是不依赖于NR。氰化物、氰酸盐、单线态氧猝灭剂、D2 O和CO2限制对TAG积累的影响与NR. C的抑制一致。dexiccataNR活性对AZ敏感。结果表明，AZ诱导的TAG积累的结果主要来自NR的光灭活。
Sodium azide (Az) induces accumulation of triglycerides (TAG) in the green alga Chlorelladesiccatawith minimal growth retardation (Rachutin-Zalogin & Pick, joint manuscript). To clarify the mechanism of this effect, the involvement of respiration, production of reactive oxygen species (ROS) and suppression of nitrate reductase (NR) was investigated. Different respiratory inhibitors induced minor or no TAG accumulation, ruling out respiration as the primary Az target. ROS generators failed to induce massive TAG accumulation, but the singlet oxygen quencher DABCO inhibited Az-induced TAG biosynthesis and this inhibition was suppressed in 30% D2O. Az-induced TAG accumulation was observed in nitrate, but not in ammonium medium, in which growth is not dependent on NR. Effects of cyanide, cyanate, a singlet oxygen quencher, D2O and of CO2limitation on TAG accumulation are consistent with inhibition of NR.C. desiccataNR activity is Az-sensitive. The results suggest that Az-induced TAG accumulation results primarily from photoinactivation of NR.