Hepatitis E-related adverse pregnancy outcomes and their prevention by hepatitis E vaccine in a rabbit model
Hepatitis E-related adverse pregnancy outcomes and their prevention by hepatitis E vaccine in a rabbit model
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兔模型中戊型肝炎相关的不良妊娠结局及其戊型肝炎疫苗的预防
DOI：
10.1080/22221751.2019.1643260
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发表时间：
2019-07-24
影响因子：
13.2
通讯作者：
Wang, Ling
中科院分区：
文献类型：
--
作者：
Li, Manyu;Li, Shuangshuang;Wang, Ling
戊型肝炎病毒（HEV）可导致妊娠期间的高死亡率。目的探讨不同HEV基因型对家兔妊娠的不良结局及HEV 239疫苗的预防作用。选取42只雌性家兔，随机平均分为7组（A-G）。E组（10 μ gx2）、F组（5 μ gx2）和G组（1mL PBSx2）在交配前注射HEV 239疫苗和安慰剂。妊娠后，A、E、F、G组接种1mL 1.5 × 106拷贝/mL兔HEV3， B/C组接种猪HEV4/人HEV3， D组为阴性对照。监测抗HEV抗体、HEV RNA和谷丙转氨酶(ALT)/天冬氨酸转氨酶（AST）水平。感染HEV的妊娠兔表现出粪病毒脱落、ALT/AST升高、组织病理学改变和不良妊娠结局等HEV感染症状。E组和F组免疫孕兔未出现HEV感染症状和不良后果。接种/未接种疫苗的孕兔产下的新生兔无/有戊型肝炎病毒感染症状。本研究表明，多种基因型的HEV感染可引起不良后果，HEV 239疫苗可预防妊娠兔的HEV相关不良后果。
Hepatitis E virus (HEV) can lead to high mortality during pregnancy. This study was to investigate the adverse pregnancy outcomes caused by different HEV genotypes and their prevention by HEV 239 vaccine in rabbits. Forty-two female rabbits were randomly and equally divided into 7 groups (A-G). HEV 239 vaccine and a placebo were administered to groups E (10 mu gx2), F (5 mu gx2) and G (1mL of PBSx2) before copulation. After pregnancy, 1mL of 1.5x106 copies/mL rabbit HEV3 was inoculated to groups A, E, F and G, swine HEV4/human HEV3 to groups B/C, and group D was a negative control. Anti-HEV antibody, HEV RNA, and alanine aminotransferase (ALT)/aspartate aminotransferase (AST) levels were monitored. Pregnant rabbits infected by HEV manifested HEV infection symptoms including fecal virus shedding, ALT/AST elevation, and histopathological changes, and adverse pregnancy outcomes. Immunized pregnant rabbits in groups E and F showed no HEV infection symptoms and adverse outcomes. The newborn rabbits delivered by pregnant rabbits with/without immunization showed without/with HEV infection symptoms. This study demonstrated that multiple genotypes of HEV infection can cause adverse outcomes and HEV 239 vaccine can prevent HEV-related adverse outcomes in pregnant rabbits.