Osteogenic effect of hyaluronic acid sodium salt in the pores of a hydroxyapatite scaffold
Osteogenic effect of hyaluronic acid sodium salt in the pores of a hydroxyapatite scaffold
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DOI：
10.1016/j.msec.2006.05.014
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发表时间：
2007-03
期刊：
影响因子：
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通讯作者：
M. Yoshikawa;N. Tsuji;T. Toda;H. Ohgushi
中科院分区：
文献类型：
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作者：
M. Yoshikawa;N. Tsuji;T. Toda;H. Ohgushi
根据之前的报道，体内支架孔隙中的骨再生需要大量的骨髓细胞（1×107细胞/ml）。我们推测，将多孔羟基磷灰石 (HA) 支架浸入透明质酸溶液中将促进支架中骨髓细胞密度为 1×106 个细胞/ml 的骨形成。将细胞浸泡在透明质酸溶液或培养基中后，分别接种到中心空心的圆柱形HA支架的孔中。将支架植入大鼠的背侧皮下组织中 4 周。此后，制作连续切片的石蜡标本并进行组织学观察。通过浸泡在透明质酸溶液中，在HA支架的许多孔中观察到骨形成。然而，通过浸没在培养基中，支架中没有或很少有新骨形成的孔。细胞在有或没有透明质酸的情况下进行体外培养。有和没有透明质酸的体外骨形成没有显着差异。这项研究结果表明，透明质酸与三维结构壁上的细胞结合，有效促进新骨形成。
According to previous reports, a large volume of bone marrow cells (1×107cells/ml) is required for bone regeneration in the pores of a scaffold in vivo. We theorized that immersion of a porous hydroxyapatite (HA) scaffold in hyaluronic acid solution would facilitate bone formation in the scaffold at 1×106cells/ml density of bone marrow cells. The cells were respectively seeded into pores of the cylindrical HA scaffolds with a hollow center after immersion in hyaluronic acid solution or in culture medium. The scaffolds were implanted in the dorsal subcutis of rats for 4 weeks. Thereafter, serially sectioned paraffin specimens were made and observed histologically. Bone formation was observed in many pores of HA scaffold by immersion in hyaluronic acid solution. However, there were no or less pores with new bone formation in the scaffold by immersion in culture medium. The cells were cultured with and without hyaluronic acid in vitro. There was no significant difference in bone formation in vitro with and without hyaluronic acid. The results of this study suggest that hyaluronic acid binds to the cells on the wall of three-dimensional structure and effectively promotes new bone formation.