Cell-free Protein Synthesis in a Microchamber Revealed the Presence of an Optimum Compartment Volume for High-order Reactions
Cell-free Protein Synthesis in a Microchamber Revealed the Presence of an Optimum Compartment Volume for High-order Reactions
复制标题
微室中的无细胞蛋白质合成揭示了高阶反应的最佳隔室体积的存在
DOI：
10.1021/sb400087e
复制
发表时间：
2013
影响因子：
4.7
通讯作者：
and T. Yomo
中科院分区：
文献类型：
--
作者：
T. Okano;T. Matsuura;H. Suzuki;and T. Yomo
微机电系统（MEMS）在化学和生物化学中的应用允许在微尺度隔室中进行各种反应。在这里，我们的目的是使用玻璃微室来研究蛋白质合成的隔室大小依赖性，蛋白质合成是细胞中最重要的反应之一。通过将具有不同反应顺序的无细胞蛋白质合成系统封装在毫微微升微室中，研究了用恒定拷贝数的DNA引发的反应的室大小依赖性。我们能够以高精度观察微室中的高阶反应的特定性质，并发现使用真实的生物分子的高阶反应的最佳室体积的存在。
The application of microelectromechanical systems (MEMS) to chemistry and biochemistry allows various reactions to be performed in microscale compartments. Here, we aimed to use the glass microchamber to study the compartment size dependency of the protein synthesis, one of the most important reactions in the cell. By encapsulating the cell-free protein synthesis system with different reaction orders in femtoliter microchambers, chamber size dependency of the reaction initiated with a constant copy number of DNA was investigated. We were able to observe the properties specific to the high order reactions in microcompartments with high precision and found the presence of an optimum compartment volume for a high-order reaction using real biological molecules.