A method for producing specific antisera with small doses of immunogen.
A method for producing specific antisera with small doses of immunogen.
复制标题
一种用小剂量免疫原生产特异性抗血清的方法。
DOI：
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发表时间：
1971
影响因子：
5.8
通讯作者：
G. Ross
中科院分区：
文献类型：
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作者：
J. Vaitukaitis;J. Robbins;E. Nieschlag;G. Ross
我们用20~100μg免疫原一次免疫，成功地制备了针对人绒毛膜促性腺激素亚单位和睾酮结合物的特异性抗血清。用有限数量的纯化材料生产糖蛋白和其他激素的特异性抗血清的方法的潜力促使本报告。材料与方法人绒毛膜促性腺激素亚基CR100α和CR100β。由R.E.Canfield博士和F.Morgan博士准备(1)。将睾酮-3-(0-羧甲基)肟与牛血清白蛋白(Armour)或锁孔帽状血蓝蛋白(CalBioChem)偶联(2)。将等量的完全弗氏佐剂、免疫原的0.15M氯化钠溶液和干燥的结核杆菌(2.5 mg/ml总混合物)在Sorvall Omnimixer中以最大速度匀浆5-10分钟。取3月龄新西兰大白兔背部和四肢近端皮毛，皮内注射乳剂30~50个部位，每只动物皮下注射乳剂2毫升。在...
We have succeeded in producing specific antisera to the subunits of HCG and testosterone conjugates with one immunization of 20 to 100 μg of immunogen. The potential of the method for producing specific antisera to glycoprotein and other hormones with limited amounts of purified material prompts this report. Materials and Methods CR 100α and CR 100β, the subunits of HCG,. were prepared by Drs. R. E. Canfield and F. Morgan (1). Testosterone-3-(0-carboxymethy1) oxime was coupled to either bovine serum albumin (Armour) or keyhole limpet hemocyanin (Calbiochem) (2). Equal volumes of complete Freund's adjuvant, a 0.15 M NaCl solution of the immunogen and dried tubercle bacilli (2.5 mg/ml of total mixture) were homogenized for 5–10 minutes at increasing speeds to maximum setting in a Sorvall Omnimixer. Fur was shaved from the back and proximal limbs of 3 month old New Zealand white rabbits and the emulsion administered intradermally at 30–50 sites so that each animal received a total of 2 ml of the emulsion. At...