Analysis of pancreatic endocrine development in GDF11-deficient mice
Analysis of pancreatic endocrine development in GDF11-deficient mice
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DOI：
10.1002/dvdy.20953
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发表时间：
2006-11-01
影响因子：
2.5
通讯作者：
Serup, Palle
中科院分区：
文献类型：
--
作者：
Dichmann, Darwin S.;Yassin, Hani;Serup, Palle
在这里，我们研究了GDF11在胰腺发育中的作用。通过原位杂交和逆转录-聚合酶链反应分析，我们发现Gdf11转录本在胚胎胰腺上皮继发性转变之前表达，但在继发性转变之后迅速下降。为了确定GDF11在胰腺发育中的功能，我们分析了GDF11（-/-）小鼠胚胎。在这种胚胎中，胰腺大小在胚胎日(E) 18时与野生型幼崽相比缩小了两倍。不同组织室的定量显示特定的外分泌室发育不全，而内分泌和导管室未受影响。值得注意的是，NGN3（+）内分泌前体细胞在E18时增加了4倍，尽管这些动物胰腺内分泌细胞的数量与野生型幼崽相比没有变化。同样，内分泌细胞的成熟以及α -细胞和β -细胞之间的比例也正常。
Here, we examine the role of GDF11 in pancreatic development. Using in situ hybridization and reverse transcriptase-polymerase chain reaction analyses, we show that Gdf11 transcripts are expressed in embryonic pancreas epithelium before the secondary transition but decrease rapidly afterward. To determine the function of GDF11 during pancreas development, we analyzed Gdf11(-/-) mouse embryos. In such embryos, pancreas size is twofold reduced at embryonic day (E) 18 compared with wild-type littermates. Quantification of the different tissue compartments shows a specific hypoplasia of the exocrine compartment, while the endocrine and ductal compartments are unaffected. Notably, NGN3(+) endocrine precursor cells are increased fourfold at E18, although the amount of endocrine cells in the pancreas of these animals is unchanged compared with wild-type littermates. Similarly, the maturation of endocrine cells as well as the ratio between alpha- and beta-cells appears normal.