The proportion of CD19+CD24hiCD27+ regulatory B cells predicts the occurrence of acute allograft rejection in liver transplantation
The proportion of CD19+CD24hiCD27+ regulatory B cells predicts the occurrence of acute allograft rejection in liver transplantation
复制标题
CD19( )CD24(hi)CD27( )调节性B细胞比例预测肝移植中急性同种异体移植排斥反应的发生
DOI：
10.21037/atm.2019.08.05
复制
发表时间：
2019-09-01
影响因子：
--
通讯作者：
Xia, Yongxiang
中科院分区：
文献类型：
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作者：
Zhou, Haomiug;Zhan, Feng;Xia, Yongxiang
背景
调节性B细胞(Bregs)在炎症和移植耐受中起重要作用。几项研究已经报道了移植排斥反应的肾移植患者Bregs的数量减少。然而，人们对它们在肝脏同种异体反应中的作用知之甚少。
方法
为探讨循环Bregs是否与肝移植患者的急性排斥反应(AR)有关，对19例心脏死亡(DCD)供者接受同种异体肝移植的患者进行了回顾性研究。
结果
采用流式细胞术(FCM)检测AR患者(AR组)和其他移植肝功能稳定患者(SF组)术后外周血中CD19+CD24hiCD38和CD19+CD24hiCD27+Memory Bregs的比例。结果显示，虽然SF组所有时间点的tBreg和mBreg的比例没有明显变化，但AR组的mBregs比例显著降低。所有5名AR患者对治疗反应良好，抗排斥治疗后mBregs比例显著增加。此外，4例受者被怀疑为AR，但逐渐被诊断为溶血性或梗阻性黄疸，mBregs的比例没有下降。
结论
第一次，我们的结果提示循环中mBregs比例降低在预测肝移植后AR患者中的潜在作用。
Background
Regulatory B cells (Bregs) play an essential role in inflammation and transplant tolerance. Several studies have reported a decreased number of Bregs in renal transplant patients with graft rejection. However, little is known about their role in the liver alloresponse.
Methods
To investigate whether the circulating Bregs have been associated with acute allograft rejection (AR) in liver transplantation patients, 19 patients receiving liver allografts from donation after cardiac death (DCD) donors were retrospectively studied.
Results
The postoperative proportions of circulating CD19+CD24hiCD38hi transitional Bregs (tBregs) and CD19+CD24hiCD27+ memory Bregs (mBregs) in patients diagnosed with AR (AR group) and other patients with stable allograft liver function (SF group) were evaluated using flow cytometry (FCM) analysis. Results showed that while no significant changes were found regarding both the tBreg and mBreg, proportions across all time points in the SF group, the AR group showed significantly decreased proportions of mBregs. All of the five AR patients responded fine to the treatments, and the proportions of mBregs increased significantly after anti-rejection therapies. In addition, AR was suspected in four recipients, but gradually they were diagnosed with hemolytic or obstructive jaundice and showed no decrease in the proportion of mBregs.
Conclusions
For the first time, our results suggested the potential role of a decreased proportion of circulating mBregs in predicting AR in patients with post liver transplantation.