microRNA expression profiling on individual breast cancer patients identifies novel panel of circulating microRNA for early detection.
microRNA expression profiling on individual breast cancer patients identifies novel panel of circulating microRNA for early detection.
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DOI：
10.1038/srep25997
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发表时间：
2016-05-16
影响因子：
4.6
通讯作者：
Alajez NM
中科院分区：
文献类型：
--
作者：
Hamam R;Ali AM;Alsaleh KA;Kassem M;Alfayez M;Aldahmash A;Alajez NM
乳腺癌(BC)是女性最常见的癌症类型，也是女性癌症相关死亡的第二大原因。因此，更好地了解乳腺癌的肿瘤生物学和识别新的生物标志物对于早期诊断、更好的疾病分层和治疗选择是至关重要的。在此，我们开发了一种新的方法，该方法依赖于通过使用速度真空浓缩的浓缩步骤来分离循环中的microRNA，从而导致microRNA丰度增加5倍。对公元前23年和9名正常人的单个样本进行的全球miRNA微阵列表达谱分析发现，在BC患者中有18个上调的miRNAs(p(Corr) <； 0.05)。随后用qRT-PCR在46例BC和14名对照中验证了9个miRNAs(hsa-miR-4270、hsa-miR-1225-5p、hsa-miR-188-5p、hsa-miR-1202、hsa-miR-4281、hsa-miR-1207-5p、hsa-miR-642B-3p、hsa-miR-1290和hsa-miR-3141)。这些microRNAs在I、II和III期患者中的表达总体上高于IV期患者，有可能用于早期检测。在HER2和TN中，该microRNA组的表达略高于腔内亚型患者。因此，我们开发了一种新的方法，该方法导致了一种新的microRNA小组的鉴定，该小组在BC患者中上调，具有潜在的疾病诊断和分层应用。
Breast cancer (BC) is the most common cancer type and the second cause of cancer-related death among women. Therefore, better understanding of breast cancer tumor biology and the identification of novel biomarkers is essential for the early diagnosis and for better disease stratification and management choices. Herein we developed a novel approach which relies on the isolation of circulating microRNAs through an enrichment step using speed-vacuum concentration which resulted in 5-fold increase in microRNA abundance. Global miRNA microarray expression profiling performed on individual samples from 23 BC and 9 normals identified 18 up-regulated miRNAs in BC patients (p(corr) < 0.05). Nine miRNAs (hsa-miR-4270, hsa-miR-1225-5p, hsa-miR-188-5p, hsa-miR-1202, hsa-miR-4281, hsa-miR-1207-5p, hsa-miR-642b-3p, hsa-miR-1290, and hsa-miR-3141) were subsequently validated using qRT-PCR in a cohort of 46 BC and 14 controls. The expression of those microRNAs was overall higher in patients with stage I, II, and III, compared to stage IV, with potential utilization for early detection. The expression of this microRNA panel was slightly higher in the HER2 and TN compared to patients with luminal subtype. Therefore, we developed a novel approach which led to the identification of a novel microRNA panel which was upregulated in BC patients with potential utilization in disease diagnosis and stratification.