Genetic and physical mapping of qHY-8, a pleiotropic QTL for heading date and yield-related traits in rice
Genetic and physical mapping of qHY-8, a pleiotropic QTL for heading date and yield-related traits in rice
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DOI：
10.1007/s10681-011-0581-0
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发表时间：
2012-03-01
期刊：
影响因子：
1.9
通讯作者：
Zhang, Zhi-Hong
中科院分区：
文献类型：
--
作者：
Cai, Hai-Ya;Diao, Shu;Zhang, Zhi-Hong
一个控制水稻抽穗期和产量相关性状的QTL qHY-8已被解析为一个孟德尔位点。QTL的三种基因型在BC 5 F2或BC 4F 2群体中的性状表现（以特青或粤台B为轮回亲本）表明，Lemont qHY-8基因座上的（供体亲本）等位基因对抽穗期、株高、穗长、QTL的加性效应因遗传力的不同而异，对穗粒数的加性效应最大，遗传力为98%，对穗粒数的加性效应次之，遗传力为70- 87%，其次是PH，遗传力为56- 61%，最后是SPP，遗传力为45- 47%。此外，该QTL的显性效应较弱，仅在HD和PL上显著。利用BC 5 F2群体，通过隐性分类分析，首次将qHY-8定位到299 kb的基因组区域。然后，利用一组片段置换系，置换作图将qHY-8定位在标记ID 3468和ID 4891之间的染色体区域内，对应于日本晴基因组序列上的31.4kb。对编码组蛋白样CCAAT盒结合转录因子的ORF Os 08 g 07740的序列分析表明，该ORF将暂时被称为第一个强候选基因。这些结果表明，qHY-8是一个控制抽穗期和多个产量相关性状的多效QTL，对水稻生产力的提高具有重要价值。
A QTL, qHY-8, has been resolved into a single Mendelian locus controlling heading date and yield-related traits in rice. The trait-performance of the three genotypes for the QTL in the BC5F2 or BC4F2 populations (with cultivar Teqing or YuetaiB as recurrent parents) indicated that the Lemont (donor parent) allele at qHY-8 locus showed a positive and pleiotropic additive effect on heading date (HD), plant height (PH), panicle length (PL), and spikelets per panicle (SPP).The QTL's additive effects varied for different traits according to its heritabilities, it showed a largest effect on HD with a heritability of 98%, secondly on PL with a heritability of 70-87%, then on PH with a heritability of 56-61%, and lastly on SPP with a heritability of 45-47%. In addition, the QTL showed a weaker dominant effect, significant only on HD and PL. With an advanced BC5F2 population, qHY-8 was firstly mapped to a 299 kb genomic region by recessive-class analysis. Then, with a set of segment substitution lines, substitution mapping located qHY-8 within a chromosomal region between markers ID3468 and ID4891, corresponding to 31.4 kb on the genome sequence of Nipponbare. Sequence analysis of an ORF Os08g07740 which encodes a histone-like CCAAT-box binding transcription factor suggested that the ORF would be tentatively referred to as the first strong candidate gene. All these results demonstrated that qHY-8 is a pleiotropic QTL for heading date and multiple yield-related traits and would be of great value to the improvement of rice productivity.