The isolation of cytoplasmic polyhedrosis virus from the white-marked tussock moth, Orgyia leucostigma (Smith).
The isolation of cytoplasmic polyhedrosis virus from the white-marked tussock moth, Orgyia leucostigma (Smith).
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从白斑草蛾 Orgyia leucostigma (Smith) 中分离出细胞质多角体病毒。
DOI：
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发表时间：
1970
期刊：
影响因子：
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通讯作者：
F. T. Bird
中科院分区：
文献类型：
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作者：
Y. Hayashi;F. T. Bird
对游离型和封闭型胞质型多角体病毒的分离纯化方法进行了改进。病毒体对核糖核酸酶、脱氧核糖核酸酶、胰蛋白酶、胰凝乳蛋白酶和磷脂酶C以及去污剂脱氧胆酸盐具有抗性。使用这些酶和脱氧胆酸盐，结合不连续和线性蔗糖梯度，产生非常纯的游离病毒体制剂。通过在室温下将多角体溶解在0.05M Na 2CO 3 - 0.04M NaCl中60分钟来释放封闭的病毒体。 病毒粒子的组成约为28% RNA和72%蛋白质。
The procedure for isolating and purifying free and occluded cytoplasmic polyhedrosis virions was improved. The virions are resistant to the enzymes RNase, DNase, trypsin, chymotrypsin, and phospholipase C, and to a detergent, deoxycholate. The use of these enzymes and deoxycholate, in combination with discontinuous and linear sucrose gradients, produced very pure preparations of free virions. Occluded virions were released by dissolving polyhedra in 0.05 M Na2CO3 – 0.04 M NaCl for 60 min at room temperature. The composition of the virion was estimated to be about 28% RNA and 72%protein.