Ionic strength effects on electrophoretic focusing and separations
Ionic strength effects on electrophoretic focusing and separations
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DOI：
10.1002/elps.200900560
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发表时间：
2010-03-01
期刊：
影响因子：
2.9
通讯作者：
Santiago, Juan G.
中科院分区：
文献类型：
--
作者：
Bahga, Supreet S.;Bercovici, Moran;Santiago, Juan G.
本文对离子强度对电泳法聚焦和分离的影响进行了数值和实验研究。我们回顾了有关离子迁移率和化学活性的离子强度模型的发展，并强调了它们在电泳分离和聚焦模拟方面的差异。我们耦合了一个用于电泳传输的快速数值求解器、用于实际离子迁移率的Onsager-Fuoss模型和用于修正离子活动性的扩展的DebyHuckle理论。对于荧光素迁移率作为离子强度和pH的函数的模型预测与CZE实验的数据进行了很好的比较。用ITP实验研究了离子强度对同时聚焦和分离的影响，并与10 mm和250 mm离子强度下的模拟结果进行了比较，结果表明，该模型能够捕捉到ITP分析物区域形状和顺序的定性差异。最后，我们给出了CZE实验的模拟结果，其中离子强度的变化会导致分析物峰的选择性和顺序的显著变化。我们对毛细管电泳中离子强度效应的模拟结果与已发表的实验数据进行了比较
We present a numerical and experimental study of the effects of ionic strength on electrophoretic focusing and separations. We review the development of ionic strength models for electrophoretic mobility and chemical activity and highlight their differences in the context of electrophoretic separation and focusing simulations We couple a fast numerical solver for electrophoretic transport with the Onsager-Fuoss model for actual ionic mobility and the extended Debye-Huckle theory for correction of ionic activity Model predictions for fluorescein mobility as a function of ionic strength and pH compare well with data from CZE experiments. Simulation predictions of preconcentration factors in peak mode ITP also compare well with the published experimental data We performed ITP experiments to study the effect of ionic strength on the simultaneous focusing and separation Our comparisons of the latter data With Simulation results at 10 and 250 mM ionic strength show the model is able to capture the observed qualitative differences in ITP analyte zone shape and order Finally, we present simulations of CZE experiments where changes in the ionic strength result in significant change in selectivity and order of analyte peaks Our simulations of ionic strength effects in capillary electrophoresis compare well with the published experimental data