Three-dimensional structure of herpes simplex virus from cryo-electron tomography
Three-dimensional structure of herpes simplex virus from cryo-electron tomography
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DOI：
10.1126/science.1090284
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发表时间：
2003-11-21
期刊：
影响因子：
56.9
通讯作者：
Steven, AC
中科院分区：
文献类型：
--
作者：
Grünewald, K;Desai, P;Steven, AC
单纯疱疹病毒是一种高度复杂的DNA病毒，由被膜（一种蛋白质区室）包围的核衣壳和包膜组成。后一种成分是多形性的，对传染性至关重要。在分离病毒粒子的低温电子断层扫描中，可以看到被膜形成一个不对称的帽状结构：在一侧，衣壳紧密靠近包膜；在另一边，它们被大约35纳米的被膜隔开。被毛亚结构呈颗粒状，有一些短的肌动蛋白状。哀叹道。包膜包含600到750个糖蛋白尖峰，这些尖峰在长度、间距和从膜上伸出的角度上都有所不同。它们的分布是非随机的，提示功能聚类。
Herpes simplex virus, a DNA virus of high complexity, consists of a nucleocapsid surrounded by the tegument-a protein compartment-and the envelope. The latter components, essential for infectivity, are pleiomorphic. Visualized in cryo-electron tomograms of isolated virions, the tegument was seen to form an asymmetric cap: On one side, the capsid closely approached the envelope; on the other side, they were separated by similar to35 nanometers of tegument. The tegument substructure was particulate, with some short actin-like. laments. The envelope contained 600 to 750 glycoprotein spikes that varied in length, spacing, and in the angles at which they emerge from the membrane. Their distribution was nonrandom, suggesting functional clustering.