Microsatellite polymorphism in Pisum sativum
Microsatellite polymorphism in Pisum sativum
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DOI：
10.1046/j.1439-0523.2001.00608.x
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发表时间：
2001-08-01
期刊：
影响因子：
2
通讯作者：
Baranger, A
中科院分区：
文献类型：
--
作者：
Burstin, J;Deniot, G;Baranger, A
从Genbank/EMBL数据库检索Pisum satium序列，搜索所有可能的二核苷酸和三核苷酸串联重复序列。在663个序列中发现了171个简单序列重复序列（SSRs），出现不同的二核苷酸或三核苷酸基序，频率不同。CT/AG是最常见的二核苷酸，TCT/AGA是最常见的三核苷酸。从这些序列中产生43个微卫星标记，并用于评估12个豌豆基因型的遗传变异。31个基因型存在多态性，仅考虑多态性标记时，每个标记的平均变异数为3.6个。总体而言，一个SSR标记的变异数与SSR长度相关，但一些12bp长的SSR与较长的SSR表现出相同程度的多态性。通过SSR基因分型对12个基因型进行分组，对一个新近栽培品种的可能起源有了有趣的认识。数据库衍生的SSR标记是高度可变的。它们可以为不同栽培类型的油菜遗传多样性提供有用的信息。
Pisum sativum sequences were retrieved from Genbank/EMBL databases and searched for all possible dinucleotide and trinucleotide tandem repeats. One-hundred and seventy-one simple sequence repeats (SSRs) were found among 663 sequences, The different dinucleotide or trinucleotide motifs occurred,It varying frequencies. CT/AG was the most frequent dinucleotide, and TCT/AGA the most frequent trinucleotide. Forty-three microsatellite markers were generated from these sequences and used to assess the genetic variability among 12 pea genotypes. Thirty-one were polymorphic among the genotypes and the average number of variants per marker was 3.6 when considering only polymorphic markers. Overall, the number of variants for a given SSR marker was correlated with the length of the SSR but some 12-bp long SSRs showed the same degree of polymorphism as longer ones. The groupings resulting from the SSR genotyping among the 12 genotypes gave an interesting insight into the possible origin of one recent cultivar. Database-derived SSR markers are highly variable. They can provide useful information on the genetic diversity among P. sativum cultivated types.