Olfactory glomeruli in the zebrafish form an invariant pattern and are identifiable across animals
Olfactory glomeruli in the zebrafish form an invariant pattern and are identifiable across animals
复制标题
斑马鱼的嗅觉肾小球形成不变的模式，并且在动物之间是可识别的
DOI：
--
复制
发表时间：
1994
影响因子：
5.3
通讯作者：
S. I. Korsching
中科院分区：
文献类型：
--
作者：
Herwig Baier;S. I. Korsching
肾小球是脊椎动物嗅球中的解剖学和可能的功能模块。本文研究了成年斑马鱼嗅球中小球的空间排列。将亲脂性示踪剂Dil的溶液注射到鼻腔中。从嗅上皮投射到球的感觉神经元的轴突顺行追踪，从而标记整个群体的肾小球。通过共聚焦激光扫描显微镜详细分析肾小球分布。我们发现，一个典型的嗅球包含少量的约80肾小球，具有定型的配置在所有的动物调查。所有肾小球均呈双侧对称性。22个单一的肾小球可以确定从动物到动物的特征性位置和形态。其余的肾小球要么嵌入肾小球丛，因此不能可靠地划定，或属于一个密集的平均49个肾小球在背嗅球群集亚群。未发现性二态性肾小球。为了测试肾小球恒定性是否是特定的斑马鱼，我们进行了类似的跟踪实验，在金鱼，并发现了几个迹象，在这个物种的肾小球类似的不变性。这种模式的显著刻板性让人联想到昆虫的嗅觉系统，并在脊椎动物中首次得到证实。现在可以检查这些可识别的肾小球是否在气味处理方面功能特化。如果是这样的话，斑马鱼可能会成为研究嗅觉编码的一个易处理的模型系统。
Glomeruli are anatomical and possibly functional modules in the vertebrate olfactory bulb. We investigated the spatial arrangement of glomeruli in the olfactory bulbs of adult zebrafish (Brachydanio rerio). A solution of the lipophilic tracer Dil was injected into the nasal cavities. Axons of sensory neurons projecting from the olfactory epithelium into the bulb were traced anterogradely, thus labeling the whole population of glomeruli. The glomerular distribution was analyzed in detail by confocal laser-scanning microscopy. We find that a typical olfactory bulb contains a small number of about 80 glomeruli that have a stereotyped configuration in all animals investigated. All glomeruli exhibit bilateral symmetry. Twenty-two single glomeruli could be identified from animal to animal by their characteristic position and morphology. The remaining glomeruli either are embedded in glomerular plexus and therefore cannot be delineated reliably, or belong to a densely clustered subpopulation of on average 49 glomeruli in the dorsal olfactory bulb. No sexually dimorphic glomeruli were identified. To test whether glomerular constancy is specific for the zebrafish, we performed similar tracing experiments in the goldfish and found several indications for a similar invariance of glomeruli in this species. The remarkable stereotypy of this pattern is reminiscent of the insect olfactory system and has been demonstrated here for the first time in a vertebrate. It will now be possible to examine whether these identifiable glomeruli are functionally specialized in terms of odor processing. If so, zebrafish may emerge as a tractable model system for studies on olfactory coding.