GROWTH-YIELD INCREASE LINKED TO CAFFEATE REDUCTION IN ACETOBACTERIUM-WOODII
GROWTH-YIELD INCREASE LINKED TO CAFFEATE REDUCTION IN ACETOBACTERIUM-WOODII
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DOI：
10.1007/bf00414460
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发表时间：
1984-01-01
影响因子：
2.8
通讯作者：
PFENNIG, N
中科院分区：
文献类型：
--
作者：
TSCHECH, A;PFENNIG, N
生长产量用A. woodii菌株NZva 16在H2 + CO2、甲酸盐、甲醇、香草酸盐、阿魏酸盐和果糖上在矿物培养基中在不存在和存在0.05%酵母提取物的情况下进行。酵母提取物对生长不是必需的，但根据发酵的底物，其生长产量提高了25-100%。比较甲酸盐或甲醇的产率，可以计算出在A. woodii。在6 mM咖啡酸盐的存在下，用底物甲酸盐或甲醇测定生长产率。咖啡因被还原为氢咖啡酸盐，并获得增加的生长产量。ATP产率约为计算1 mol/mol还原的咖啡酸盐。在无细胞浸提液或膜制备物中未检测到细胞色素。
Growth yields were determined with A. woodii strain NZva 16 on H2 plus CO2, formate, methanol, vanillate, ferulate and fructose in mineral medium in the absence and presence of 0.05% yeast extract. Yeast extract was not essential for growth but enhanced growth yields by 25-100% depending on the substrate fermented. Comparison of yields on formate or methanol allowed calculation of an energy yield in the range of 1.5-2 mol ATP/mol acetate formed during homoacetate fermentation of A. woodii. In the presence of 6 mM caffeate, growth yields were determined with the substrates formate or methanol. Caffeate was reduced to hydrocaffeate and increased growth yields were obtained. An ATP yield of .apprx. 1 mol/mol of caffeate reduced was calculated. Cytochromes were not detectable in cell free extracts or membrane preparations.