Dependence of optical scattering from Intralipid in gelatin-gel based tissue-mimicking phantoms on mixing temperature and time
Dependence of optical scattering from Intralipid in gelatin-gel based tissue-mimicking phantoms on mixing temperature and time
复制标题
DOI：
10.1117/1.jbo.19.3.035002
复制
发表时间：
2014-03-01
影响因子：
3.5
通讯作者：
Wang, Lihong V.
中科院分区：
文献类型：
--
作者：
Lai, Puxiang;Xu, Xiao;Wang, Lihong V.
脂内脂肪被广泛用作模拟组织模体的光学散射剂。使用Intralipid时的准确控制对于将模体的光学漫射率匹配到规定值至关重要。目前，基于Intralipid的水凝胶模体制作方案大多关注Intralipid品牌和浓度等因素。在本文中，我们首次探索了Intralipid与明胶-水溶液混合的温度和时间对光学约化散射系数(在532 nm处)的依赖关系。所研究的样品中含有1%的Intralipid，并用斜入射反射仪进行测量。研究发现，当Intralipid-明胶-水混合物在室温下开始固化时，折合散射系数增大。对于已经完全固化的模体，混合过程的温度和持续时间对扩散系数有显著影响。实验观测证实了测量的扩散系数与混合条件的关系。此外，还对依赖行为背后的机制进行了探讨。(C)2014年光学仪器工程师学会(SPIE)
Intralipid is widely used as an optical scattering agent in tissue-mimicking phantoms. Accurate control when using Intralipid is critical to match the optical diffusivity of phantoms to the prescribed value. Currently, most protocols of Intralipid-based hydrogel phantom fabrication focus on factors such as Intralipid brand and concentration. In this note, for the first time to our knowledge, we explore the dependence of the optical reduced scattering coefficient (at 532 nm optical wavelength) on the temperature and the time of mixing Intralipid with gelatin-water solution. The studied samples contained 1% Intralipid and were measured with oblique-incidence reflectometry. It was found that the reduced scattering coefficient increased when the Intralipid-gelatin-water mixture began to solidify at room temperature. For phantoms that had already solidified completely, the diffusivity was shown to be significantly influenced by the temperature and the duration of the mixing course. The dependence of the measured diffusivity on the mixing conditions was confirmed by experimental observations. Moreover, the mechanism behind the dependence behavior is discussed. (C) 2014 Society of Photo-Optical Instrumentation Engineers (SPIE)