Enhancing bottom-up and top-down proteomic measurements with ion mobility separations.
Enhancing bottom-up and top-down proteomic measurements with ion mobility separations.
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DOI：
10.1002/pmic.201500048
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发表时间：
2015-08
期刊：
影响因子：
3.4
通讯作者：
Smith RD
中科院分区：
文献类型：
--
作者：
Baker ES;Burnum-Johnson KE;Ibrahim YM;Orton DJ;Monroe ME;Kelly RT;Moore RJ;Zhang X;Théberge R;Costello CE;Smith RD
具有更高通量、灵敏度和结构信息的蛋白质组学测量对于改善复杂混合物的深入表征和有针对性的研究至关重要。虽然LC分离与MS（LC-MS）测量相结合提供了不同样品类型中数千种蛋白质的信息，但引入分离阶段可提供进一步的组分分辨率和快速的结构信息，这在蛋白质组学分析中具有许多优势。离子传输和数据采集方面的技术进步使得离子迁移率分离成为一种合适的技术，可以轻松有效地结合到LC-MS蛋白质组学测量中，以提高其信息含量。在这里，我们报告的应用程序，说明增加的灵敏度，吞吐量和结构信息，利用IMS-MS和LC-IMS-MS测量自下而上和自上而下的蛋白质组学测量。
Proteomic measurements with greater throughput, sensitivity, and structural information are essential for improving both in-depth characterization of complex mixtures and targeted studies. While LC separation coupled with MS (LC–MS) measurements have provided information on thousands of proteins in different sample types, the introduction of a separation stage that provides further component resolution and rapid structural information has many benefits in proteomic analyses. Technical advances in ion transmission and data acquisition have made ion mobility separations an opportune technology to be easily and effectively incorporated into LC–MS proteomic measurements for enhancing their information content. Herein, we report on applications illustrating increased sensitivity, throughput, and structural information by utilizing IMS–MS and LC–IMS–MS measurements for both bottom-up and top-down proteomics measurements.