Association between Chronic Atrophic Gastritis and Serum Antibodies to 15 Helicobacter pylori Proteins Measured by Multiplex Serology
Association between Chronic Atrophic Gastritis and Serum Antibodies to 15 Helicobacter pylori Proteins Measured by Multiplex Serology
复制标题
DOI：
10.1158/0008-5472.can-08-3477
复制
发表时间：
2009-04-01
期刊：
影响因子：
11.2
通讯作者：
Brenner, Hermann
中科院分区：
文献类型：
--
作者：
Gao, Lei;Weck, Melanie N.;Brenner, Hermann
幽门螺杆菌感染是慢性萎缩性胃炎（CAG）的主要危险因素，CAG是肠胃癌的前病变。细菌的致病性被认为在决定临床结果的范围和严重程度方面发挥着重要作用。我们的目的是评估CAG和15 H抗体血清状态之间的关系。pylori蛋白这些分析基于534例血清学确诊的萎缩性胃炎病例和1，068名年龄和性别匹配的对照，这些病例和对照参与了在德国萨尔兰进行的一项基于人群的研究，研究对象为9，953名50至74岁的男性和女性。新开发的H. pylori多重血清学方法检测15 H抗体。pylori抗原。在所有15种特异性抗体的血清阳性与CAG的存在之间观察到显著相关性。排除严重病例（可能在CAG进展过程中失去感染），大大增加了观察到的相关性。In H.幽门螺杆菌血清阳性受试者、细胞毒素相关基因A（CagA）、空泡毒素（VacA）、富含半胱氨酸的Helicobacter蛋白C（HcpC）和伴侣蛋白GroEL被确定为CAG的独立毒力因子，校正比值比（95%置信区间）分别为3.52（2.01-6.10）、3.19（1.44-7.05）、4.03（1.53-10.65）和2.65（1.06-6.62）;同时存在所有四种独立毒力因子与CAG风险增加18倍相关。结论：HcpC和GroEL是新的独立毒力因子，与CagA和VacA毒力因子联合作用与CAG密切相关。[Cancer Res 2009;69（7）：2973-80]
Infection with Helicobacter pylori is a major risk factor for chronic atrophic gastritis (CAG), a precursor lesion of intestinal gastric cancer. The pathogenicity of the bacterium is thought to play an important role in determining the extent and severity of clinical outcome. We aimed to assess the associations between CAG and the serostatus of antibodies to 15 H. pylori proteins. The analyses were based on 534 cases with serologically defined CAG and 1,068 age-matched and sex-matched controls participating in a population-based study conducted in Saarland, Germany among 9,953 men and women ages 50 to 74 years. A newly developed H. pylori multiplex serology method was used to detect antibodies specific to 15 H. pylori antigens. Significant associations were observed between seropositivity for all 15 specific antibodies and the presence of CAG. Exclusion of severe cases, who might have lost the infection in the course of CAG progression, substantially increased the observed associations. In H. pylori-seropositive subjects, cytotoxin-associated gene A (CagA), vacuolating toxin (VacA), helicobacter cysteine-rich protein C (HcpC), and the chaperonin GroEL were identified as independent virulence factors for CAG with adjusted odds ratios (95% confidence interval) of 3.52 (2.01-6.10), 3.19 (1.44-7.05), 4.03 (1.53-10.65), and 2.65 (1.06-6.62), respectively; the simultaneous presence of all four independent virulence factors was associated with an 18-fold risk of CAG. In conclusion, HcpC and GroEL were identified as new independent virulence factors, and in combination with the established virulence factors, CagA and VacA, were strongly associated with CAG. [Cancer Res 2009;69(7):2973-80]