Rice Ovate Family Protein 2 (OFP2) alters hormonal homeostasis and vasculature development
Rice Ovate Family Protein 2 (OFP2) alters hormonal homeostasis and vasculature development
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DOI：
10.1016/j.plantsci.2015.10.011
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发表时间：
2015-12-01
期刊：
影响因子：
5.2
通讯作者：
Walia, Harkamal
中科院分区：
文献类型：
--
作者：
Schmitz, Aaron J.;Begcy, Kevin;Walia, Harkamal
OFP （Ovate Family Protein）是一个仅存在于植物中的转录因子家族。在薯蓣中，OFPs控制果实形状和次生细胞壁的生物合成。OFPs也被认为通过与KNOX和BELL转录因子的相互作用发挥作用。在这里，我们对与调节水果形状相关的OFP亚群成员OsOFP2进行了功能表征。发现OsOFP2定位于细胞核和细胞质。在OVATE区域内发现了一个假定的核输出信号，这是OsOFP2定位到不同细胞质点所必需的。过表达OsOFP2的水稻植株高度降低，叶片形态、种子形状和茎维管束位置发生改变。转录组分析表明，与脉管系统发育、木质素生物合成和激素稳态相关的基因被破坏。在OsOFP2过表达系中，赤霉素生物合成基因GA 20-氧化酶7的表达降低与赤霉素含量降低相一致。此外，我们发现OsOFP2在植物脉管系统中表达，并确定了假定的血管发育KNOX和BELL蛋白与OsOFP2相互作用。已知KNOX和BELL基因通过GA20ox基因调控抑制赤霉素的生物合成，可以限制木质素的生物合成。我们认为O5OFP2可以调节KNOX-BELL功能来控制包括脉管系统发育在内的多种发育方面。2015爱思唯尔爱尔兰有限公司版权所有。
OFP (Ovate Family Protein) is a transcription factor family found only in plants. In dicots, OFPs control fruit shape and secondary cell wall biosynthesis. OFPs are also thought to function through interactions with KNOX and BELL transcription factors. Here, we have functionally characterized OsOFP2, a member of the OFP subgroup associated with regulating fruit shape. OsOFP2 was found to localize to the nucleus and to the cytosol. A putative nuclear export signal was identified within the OVATE domain and was required for the localization of OsOFP2 to distinct cytosolic spots. Rice plants overexpressing OsOFP2 were reduced in height and exhibited altered leaf morphology, seed shape, and positioning of vascular bundles in stems. Transcriptome analysis indicated disruptions of genes associated with vasculature development, lignin biosynthesis, and hormone homeostasis. Reduced expression of the gibberellin biosynthesis gene GA 20-oxidase 7 coincided with lower gibberellin content in OsOFP2 overexpression lines. Also, we found that OsOFP2 was expressed in plant vasculature and determined that putative vascular development KNOX and BELL proteins interact with OsOFP2. KNOX and BELL genes are known to suppress gibberellin biosynthesis through GA20ox gene regulation and can restrict lignin biosynthesis. We propose that O5OFP2 could modulate KNOX-BELL function to control diverse aspects of development including vasculature development. (C) 2015 Elsevier Ireland Ltd. All rights reserved.