An evaluation of serological tests in the diagnosis of bovine brucellosis in naturally infected cattle in KwaZulu-Natal province in South Africa
An evaluation of serological tests in the diagnosis of bovine brucellosis in naturally infected cattle in KwaZulu-Natal province in South Africa
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DOI：
10.4102/jsava.v88i0.1381
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发表时间：
2017-01-01
影响因子：
0.9
通讯作者：
van Heerden, Henriette
中科院分区：
文献类型：
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作者：
Chisi, Songelwayo L.;Marageni, Yoanda;van Heerden, Henriette
用玫瑰花环试验(RBT)、补体结合试验(CFT)、血清凝集试验(SAT)、竞争性酶联免疫吸附试验(CELISA)和间接酶联免疫吸附试验(IEL ISA)对南非夸祖鲁-纳塔尔省自然感染牛(金标准)进行了诊断敏感性(DSE)、补体结合试验(CFT)、血清凝集试验(SAT)、竞争酶联免疫吸附试验(CELISA)和间接酶联免疫吸附试验(IELISA)的检测。通过从皱胃液、乳汁、湿液、淋巴结或子宫排泄物标本中培养和鉴定流产布鲁氏菌生物群1，确定动物自然感染布鲁氏菌病的情况。上述试验的诊断特异性(DSP)是使用已知阴性群体的样本确定的。在诊断布鲁氏菌病的能力方面，这些测试之间没有统计学上的显著差异。RBT和iEL ISA的DSE最高，为95.8%，RBT和CFT的DSE最高，为100%。在南非的实验室中，使用RBT和CFT血清学测试，因为与劳动力密集度较低但更昂贵的iELISA相比，CFT具有成本效益。
The diagnostic sensitivity (DSe) of the Rose Bengal test (RBT), the complement fixation test (CFT), the serum agglutination test (SAT), the competitive enzyme-linked immunosorbent assay (cELISA) and the indirect ELISA (iELISA) were determined in naturally infected cattle in KwaZulu-Natal province of South Africa with known infectious status from culture (gold standard). Natural brucellosis infection status of animals was determined by culturing and identification of Brucella abortus biovar 1 from abomasal fluid, milk, hygroma fluid, lymph nodes or uterine discharges samples. The diagnostic specificity (DSp) of the tests mentioned above was determined using samples from known negative herds. There was no statistically significant difference between the tests in their ability to diagnose brucellosis. The RBT and iELISA had the highest DSe of 95.8%, whereas RBT and CFT had the highest DSp of 100%. In South African laboratories, the RBT and CFT serological tests are used, because of the cost efficacy of CFT when compared to the less labour intensive but more expensive iELISA.