Interleukin-1 expression in inflammatory myopathies: Evidence of marked immunoreactivity in sarcoid granulomas and muscle fibres showing ischaemic and regenerative changes
Interleukin-1 expression in inflammatory myopathies: Evidence of marked immunoreactivity in sarcoid granulomas and muscle fibres showing ischaemic and regenerative changes
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DOI：
10.1111/j.1365-2990.1997.tb01195.x
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发表时间：
1997-04-01
影响因子：
5
通讯作者：
Gherardi, RK
中科院分区：
文献类型：
--
作者：
Authier, FJ;Mhiri, C;Gherardi, RK
最常见的自身免疫性成人炎症性肌病是皮肌炎、多发性肌炎、包涵体肌炎和结节性肌病。白细胞介素-1 (IL-1) 是一种多效性分子，不仅参与炎症过程，还参与组织保护和重塑。我们评估了皮肌炎（15 例）、多发性肌炎（5 例）、包涵体肌炎（5 例）和结节性肌病（5 例）患者的冷冻肌肉活检标本中 IL-1 α 和 β 的免疫细胞化学表达。在炎症细胞和肌肉纤维中均观察到阳性免疫反应性。通过蛋白质印迹实验评估免疫染色的特异性。 IL-1阳性炎症细胞在多发性肌炎和包涵体肌炎中很少见，在皮肌炎中中等丰富，在结节性肌病肉芽肿中突出。在结节性肌病中，24.6 +/- 4.1% 的炎症细胞为 IL-1 α 阳性，45.2 +/- 2.6% 的炎症细胞为 IL-1 β 阳性。 IL-1 阳性肌纤维主要见于皮肌炎，通常远离炎症浸润。在显示缺血性空泡的纤维中观察到 IL-1 阳性免疫染色，这些空泡对应于肌醇溶解区域、萎缩的束周纤维以及位于愈合微梗塞内的纤维。所有 NCAM 阳性再生纤维均为 IL-1 阳性。我们的结论是： (i) IL-1 在结节性肌病的肉芽肿中表达，这与 IL-1 在肉芽肿形成中的作用一致； (ii) IL-1由遭受缺血性损伤的肌纤维表达； (iii) 肌纤维表达的 IL-1 与肌原纤维蛋白分解和再生相关。
The most frequent autoimmune adult inflammatory myopathies are dermatomyositis, polymyositis, inclusion body myositis, and sarcoid myopathy. Interleukin-1 (IL-1) is a pleiotropic molecule, implicated in the inflammatory process, but also in tissue protection and remodelling. We evaluated the immunocytochemical expression of IL-1 alpha and beta in frozen muscle biopsy specimens from patients with dermatomyositis (15 cases), polymyositis (five cases), inclusion body myositis (five cases) and sarcoid myopathy (five cases). Positive immunoreactivities, were observed in both inflammatory cells and muscle fibres. Specificity of the immunostaining was assessed by Western blot experiments. IL-1 positive inflammatory cells were rare in polymyositis and inclusion body myositis, moderately abundant in dermatomyositis, and prominent in sarcoid myopathy granulomas. In sarcoid myopathy, 24.6 +/- 4.1% inflammatory cells were IL-1 alpha positive and 45.2 +/- 2.6% were IL-1 beta-positive. IL-1 positive muscle fibres were mainly observed in dermatomyositis, usually remote from inflammatory infiltrates. Positive immunostaining for IL-1 was observed in fibres showing ischaemic punched-out vacuoles, that correspond to areas of myosinolysis, in atrophic perifascicular fibres, and in fibres located within healing microinfarcts. All NCAM-positive regenerating fibres were IL-1 positive. We conclude that : (i) IL-1 is expressed in granulomas of sarcoid myopathy, which is in keeping with the role ascribed to IL-1 in the formation of granulomas; (ii) IL-1 is expressed by muscle fibres undergoing ischaemic damage; and (iii) IL-1 expression by muscle fibres is associated with myofibrillar protein breakdown and regeneration.