EXPRESSION CLONING OF AN ADENYLATE-CYCLASE COUPLED CALCITONIN RECEPTOR
EXPRESSION CLONING OF AN ADENYLATE-CYCLASE COUPLED CALCITONIN RECEPTOR
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DOI：
10.1126/science.1658940
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发表时间：
1991-11-15
期刊：
影响因子：
56.9
通讯作者：
GOLDRING, SR
中科院分区：
文献类型：
--
作者：
LIN, HY;HARRIS, TL;GOLDRING, SR
利用cDNA文库在猪肾上皮细胞系COS细胞中表达，克隆了一段降钙素受体互补DNA。482个氨基酸的受体对鲑鱼降钙素具有高亲和力（解离常数K(d)几乎等于6 nM），并且在功能上偶联到细胞内环磷酸腺苷（cAMP）的增加。该受体与其他已报道的G蛋白偶联受体没有序列相似性，但与甲状旁腺激素-甲状旁腺激素相关肽（PTH-PTHrP）受体同源，表明这些调节钙稳态的激素受体代表了一个新的G蛋白偶联受体家族。
A calcitonin receptor complementary DNA (cDNA) was cloned by expression of a cDNA library from a porcine kidney epithelial cell line in COS cells. The 482-amino acid receptor has high affinity for salmon calcitonin (dissociation constant K(d) almost-equal-to 6 nM) and is functionally coupled to increases in intracellular cyclic adenosine monophosphate (cAMP). The receptor shows no sequence similarity to other reported G protein-coupled receptors but is homologous to the parathyroid hormone-parathyroid hormone-related peptide (PTH-PTHrP) receptor, indicating that the receptors for these hormones, which regulate calcium homeostasis, represent a new family of G protein-coupled receptors.