Optics clustered to output unique solutions: A multi-laser facility for combined single molecule and ensemble microscopy
Optics clustered to output unique solutions: A multi-laser facility for combined single molecule and ensemble microscopy
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DOI：
10.1063/1.3635536
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发表时间：
2011-09-01
影响因子：
1.6
通讯作者：
Martin-Fernandez, Marisa L.
中科院分区：
文献类型：
--
作者：
Clarke, David T.;Botchway, Stanley W.;Martin-Fernandez, Marisa L.
光学集群输出独特的解决方案（OCTOPUS）是一个显微镜平台，结合了单分子和合奏成像方法。OCTOPUS的一个新方面是其激光激发系统，该系统由互锁的连续波和脉冲激光源的中心核心组成，发射到光纤中并通过激光组合器连接。将光纤插入壁挂式接线板，连接到相邻房间的显微镜终端站。这使得不同的终端站可以共享激光颜色和时间特性的多种定制组合，从而最大限度地减少了对激光复制的需求。这种设置在成本效益、易于操作和用户安全方面带来了显著的好处。OCTOPUS的模块化特性还有助于根据需要添加新技术，允许在新显微镜中使用现有激光器，同时保留运行设施现有部分的能力。到目前为止，相互关联的技术是多光子/多色共聚焦荧光寿命成像的几种形式的荧光共振能量转移（FRET）和时间分辨各向异性，全内反射荧光，单分子成像的单对FRET，单分子荧光偏振，粒子跟踪，和光学镊子。在这里，我们使用一个研究充分的系统，表皮生长因子受体网络，来说明OCTOPUS如何帮助调查复杂的生物现象。(C)2011年美国物理学会。[doi：10.1063/1.3635536]
Optics clustered to output unique solutions (OCTOPUS) is a microscopy platform that combines single molecule and ensemble imaging methodologies. A novel aspect of OCTOPUS is its laser excitation system, which consists of a central core of interlocked continuous wave and pulsed laser sources, launched into optical fibres and linked via laser combiners. Fibres are plugged into wall-mounted patch panels that reach microscopy end-stations in adjacent rooms. This allows multiple tailor-made combinations of laser colours and time characteristics to be shared by different end-stations minimising the need for laser duplications. This setup brings significant benefits in terms of cost effectiveness, ease of operation, and user safety. The modular nature of OCTOPUS also facilitates the addition of new techniques as required, allowing the use of existing lasers in new microscopes while retaining the ability to run the established parts of the facility. To date, techniques interlinked are multi-photon/multicolour confocal fluorescence lifetime imaging for several modalities of fluorescence resonance energy transfer (FRET) and time-resolved anisotropy, total internal reflection fluorescence, single molecule imaging of single pair FRET, single molecule fluorescence polarisation, particle tracking, and optical tweezers. Here, we use a well-studied system, the epidermal growth factor receptor network, to illustrate how OCTOPUS can aid in the investigation of complex biological phenomena. (C) 2011 American Institute of Physics. [doi:10.1063/1.3635536]