Microwave-Assisted Hydrothermal Rapid Synthesis of Calcium Phosphates: Structural Control and Application in Protein Adsorption.
Microwave-Assisted Hydrothermal Rapid Synthesis of Calcium Phosphates: Structural Control and Application in Protein Adsorption.
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微波辅助水热快速合成磷酸钙：结构控制及其在蛋白质吸附中的应用
DOI：
10.3390/nano5031284
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发表时间：
2015-07-31
期刊：
影响因子：
--
通讯作者：
Qian QR
中科院分区：
文献类型：
--
作者：
Cai ZY;Peng F;Zi YP;Chen F;Qian QR
合成磷酸钙（CaP）基材料在生物医学领域备受关注。在这项研究中，我们研究了pH值对微波辅助水热法制备的CaP纳米结构的影响。在弱酸性条件（pH 5）下制备了层次化羟基磷灰石（HAP）纳米结构，在中性条件（pH 7）和弱碱性条件（pH 9）下制备了羟基磷灰石纳米棒。当pH值增加到11时，得到HAP纳米棒与磷酸三钙纳米颗粒的混合产物。结果表明，初始反应溶液的pH值对CaP的物相和结构有重要影响。此外，以血红蛋白（Hb）为模型蛋白，研究了制备的CaP纳米结构的蛋白质吸附和释放性能。在pH = 11时制备的由纳米棒和纳米片混合形态组成的样品比在pH = 5时制备的样品具有更高的Hb蛋白吸附能力，这可能是由于其较小的尺寸和分散的结构。结果表明，制备的CaP纳米结构具有较高的蛋白质吸附能力，在药物传递和蛋白质吸附等生物医学领域具有广阔的应用前景。
Synthetic calcium phosphate (CaP)-based materials have attracted much attention in the biomedical field. In this study, we have investigated the effect of pH values on CaP nanostructures prepared using a microwave-assisted hydrothermal method. The hierarchical nanosheet-assembled hydroxyapatite (HAP) nanostructure was prepared under weak acidic conditions (pH 5), while the HAP nanorod was prepared under neutral (pH 7) and weak alkali (pH 9) condition. However, when the pH value increases to 11, a mixed product of HAP nanorod and tri-calcium phosphate nanoparticle was obtained. The results indicated that the pH value of the initial reaction solution played an important role in the phase and structure of the CaP. Furthermore, the protein adsorption and release performance of the as-prepared CaP nanostructures were investigated by using hemoglobin (Hb) as a model protein. The sample that was prepared at pH = 11 and consisted of mixed morphologies of nanorods and nanoprisms showed a higher Hb protein adsorption capacity than the sample prepared at pH 5, which could be explained by its smaller size and dispersed structure. The results revealed the relatively high protein adsorption capacity of the as-prepared CaP nanostructures, which show promise for applications in various biomedical fields such as drug delivery and protein adsorption.