Poly(D,L lactic-co-glycolic acid) microspheres as biodegradable microcarriers for pluripotent stem cells
Poly(D,L lactic-co-glycolic acid) microspheres as biodegradable microcarriers for pluripotent stem cells
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DOI：
10.1016/j.biomaterials.2004.01.027
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发表时间：
2004-11-01
期刊：
影响因子：
14
通讯作者：
McBurney, MW
中科院分区：
文献类型：
--
作者：
Newman, KD;McBurney, MW
胚胎干细胞的多能性和增殖能力使其成为组织工程和再生的有吸引力的细胞来源。在我们的研究中，我们研究了聚（D， l -乳酸-羟基乙酸）（PLGA）微球作为多能细胞的可生物降解微载体和影响细胞分化的生物活性因子的传递系统。以多能性P19胚胎癌细胞系为模型，研究了多能干细胞在PLGA微球上的细胞附着、生长和分化。维甲酸（Retinoic acid， RA）被包裹在PLGA微载体中，以影响细胞分化，更具体地说，是诱导P19细胞向神经元分化。结果表明，P19细胞在负载RA的PLGA微球表面附着生长。此外，负载RA的PLGA微球在诱导P19细胞向神经元分化方面与可溶性RA一样有效。因此，这些体外研究的结果清楚地证明了PLGA微球作为生物活性因子的递送系统和多能细胞的支架的双重作用。更重要的是，我们的研究证明了PLGA微球作为多能干细胞移植基质在组织工程和再生中的潜在应用。(C) 2004 Elsevier Ltd.版权所有。
The pluripotent nature and proliferative capacity of embryonic stem cells makes them an attractive cell source for tissue engineering and regeneration. In our study we investigated the use of poly(D,L-lactic-co-glycolic acid) (PLGA) microspheres as biodegradable microcarriers of pluripotent cells and as delivery systems of bioactive factors, which influence cell differentiation. The pluripotent P19 embryonal carcinoma cell line was used as a model to study cell attachment, growth and differentiation of pluripotent stem cells on PLGA microspheres. Retinoic acid (RA) was encapsulated in the PLGA microcarriers to influence cell differentiation-m ore specifically, to induce P19 cell differentiation into neurons. The results revealed that P19 cells attach and grow on the surface of the RA loaded PLGA microspheres. Moreover, the RA loaded PLGA microspheres were shown to be as effective as soluble RA at inducing P19 cell differentiation into neurons. Hence, the results of these ex vivo studies clearly demonstrate the capacity of PLGA microspheres to serve a dual role as both delivery systems of bioactive factors and as scaffolds for pluripotent cells. More importantly, our study demonstrates the potential use of PLGA microspheres as transplantation matrices of pluripotent stem cells for tissue engineering and regeneration. (C) 2004 Elsevier Ltd. All rights reserved.