gld-1, a tumor suppressor gene required for oocyte development in Caenorhabditis elegans.
gld-1, a tumor suppressor gene required for oocyte development in Caenorhabditis elegans.
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DOI：
10.1093/genetics/139.2.579
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发表时间：
1995-02
期刊：
影响因子：
3.3
通讯作者：
Ross Francis;M. Barton;J. Kimble;T. Schedl
中科院分区：
文献类型：
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作者：
Ross Francis;M. Barton;J. Kimble;T. Schedl
我们已经鉴定了秀丽隐杆线虫的gld-1（种系发育缺陷）基因的31个突变。在gld-1（零）雌雄同体中，卵子发生被取消，在正常发生卵母细胞发育的地方形成种系肿瘤。相比之下，gld-1（零）雄性则不受影响。雌雄同体生殖系肿瘤似乎源于生殖细胞正常进入减数分裂途径，但随后退出粗线期并返回有丝分裂周期。某些gld-1部分功能丧失突变也会破坏卵子发生，但生殖细胞停留在粗线期而不是恢复有丝分裂。我们的研究结果表明，gld-1是卵母细胞发育所需的肿瘤抑制基因。肿瘤表型表明，gld-1（+）可能在减数分裂前期负调控增殖和/或在减数分裂前期直接作用。我们还发现，gld-1（+）在生殖系性别决定中还有一个非必要的作用：促进雌雄同体精子的发生。gld-1的这种功能是从单倍体不足表型和功能获得性gld-1突变的特性推断出来的，这些突变会导致生殖细胞性别身份的改变。
We have characterized 31 mutations in the gld-1 (defective in germline development) gene of Caenorhabditis elegans. In gld-1 (null) hermaphrodites, oogenesis is abolished and a germline tumor forms where oocyte development would normally occur. By contrast, gld-1 (null) males are unaffected. The hermaphrodite germline tumor appears to derive from germ cells that enter the meiotic pathway normally but then exit pachytene and return to the mitotic cycle. Certain gld-1 partial loss-of-function mutations also abolish oogenesis, but germ cells arrest in pachytene rather than returning to mitosis. Our results indicate that gld-1 is a tumor suppressor gene required for oocyte development. The tumorous phenotype suggests that gld-1(+) may function to negatively regulate proliferation during meiotic prophase and/or act to direct progression through meiotic prophase. We also show that gld-1(+) has an additional nonessential role in germline sex determination: promotion of hermaphrodite spermatogenesis. This function of gld-1 is inferred from a haplo-insufficient phenotype and from the properties of gain-of-function gld-1 mutations that cause alterations in the sexual identity of germ cells.