Video-Rate Confocal Microscopy for Single-Molecule Imaging in Live Cells and Superresolution Fluorescence Imaging
Video-Rate Confocal Microscopy for Single-Molecule Imaging in Live Cells and Superresolution Fluorescence Imaging
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DOI：
10.1016/j.bpj.2012.09.014
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发表时间：
2012-10-17
影响因子：
3.4
通讯作者：
Hohng, Sungchul
中科院分区：
文献类型：
--
作者：
Lee, Jinwoo;Miyanaga, Yukihiro;Hohng, Sungchul
目前还没有针对活细胞单分子成像和超分辨率荧光成像进行优化的共焦显微镜。据我们所知，通过结合线扫描方法的快速性和宽视野检测的高灵敏度，我们开发了一种新型共焦荧光显微镜，具有良好的光学切片能力（1.0μm）、快速的帧速率（
There is no confocal microscope optimized for single-molecule imaging in live cells and superresolution fluorescence imaging. By combining the swiftness of the line-scanning method and the high sensitivity of wide-field detection, we have developed a, to our knowledge, novel confocal fluorescence microscope with a good optical-sectioning capability (1.0 mu m), fast frame rates (