Uridine diphosphate glucosyltransferases are involved in spinosad resistance in western flower thrips Frankliniella occidentalis (Pergande)
Uridine diphosphate glucosyltransferases are involved in spinosad resistance in western flower thrips Frankliniella occidentalis (Pergande)
复制标题
DOI：
10.1016/j.jhazmat.2024.133575
复制
发表时间：
2024-01-26
影响因子：
13.6
通讯作者：
Wu，Qingjun
中科院分区：
文献类型：
--
作者：
Wang，Jing;Wan，Yanran;Wu，Qingjun
尿苷二磷酸葡萄糖基转移酶（UGTs）在昆虫解毒系统中起着至关重要的作用，并与农药抗性有关。我们之前对spinosad易感（Ivf03）和耐药（NIL-R）Frankliniella western的转录组学分析显示，NIL-R菌株中有许多UGT基因上调，表明它们可能对spinosad耐药有贡献。为了验证这一假设，我们进行了UGT活性测定和spinosad诱导实验，采用RNA干扰（RNAi）技术进行基因功能验证。我们发现，与Ivf03相比，NIL-R菌株的UGT活性显著升高，5-硝尿嘧啶对抗性菌株表现出显著的协同作用。8个UGT基因被鉴定出来，在UGT466、467和468家族中存在扩增和重复。18个ugt中有10个在NIL-R中表现出较高的表达水平，特别是fougt466b1、FoUGT468A3和fougt468a4在雌雄同体、雌雄同体中持续上调。针对这三个UGT基因的基于rnai的功能验证导致对spinosad的易感性以生命阶段、性别和剂量依赖的方式增加。这些结果表明，ugt确实参与了西方人的脊髓糖甙抗性，并且其作用与生命阶段、性别和剂量有关。因此，可持续控制f。西方的抵抗应该始终考虑到这些不同的反应。
Uridine diphosphate glucosyltransferases (UGTs) play crucial roles in the insect detoxification system and are associated with pesticide resistance. Our previous transcriptomic analysis of spinosad-susceptible (Ivf03) and resistant (NIL-R)Frankliniella occidentalisrevealed numerous upregulated UGT genes in the NIL-R strain, suggesting their potential contribution to spinosad resistance. To investigate this hypothesis, here we conducted UGT activity assays and spinosad induction experiments, employing RNA interference (RNAi) techniques for gene function validation. We found significantly elevated UGT activity in the NIL-R strain compared to Ivf03, with 5-nitrouracil showing a substantial synergistic effect on the resistant strain. Eighteen UGT genes were identified inF. occidentalis, with gene expansion and duplication observed within families UGT466, 467, and 468. Ten out of the eighteenUGTsexhibited higher expression levels in NIL-R, specificallyFoUGT466B1,FoUGT468A3, andFoUGT468A4consistently being upregulated across nymphs, males, and females. RNAi-based functional validation targeting these three UGT genes led to increased susceptibility to spinosad in a life stage-, sex-, and dose-dependent manner. These results indicate that UGTs are indeed involved in spinosad resistance inF. occidentalis, and the effects are dependent on life stage, sex, and dose. Therefore, sustainable control forF. occidentalisresistance should always consider these differential responses.