A quantitative infrared determination of acyl chain conformation in gramicidin/dipalmitoylphosphatidylcholine mixtures.
A quantitative infrared determination of acyl chain conformation in gramicidin/dipalmitoylphosphatidylcholine mixtures.
复制标题
短杆菌肽/二棕榈酰磷脂酰胆碱混合物中酰基链构象的定量红外测定。
DOI：
10.1016/0006-291x(90)91678-l
复制
发表时间：
1990
影响因子：
3.1
通讯作者：
Mendelsohn,R
中科院分区：
文献类型：
--
作者：
Davies,MA;Brauner,JW;Schuster,HF;Mendelsohn,R
用红外光谱定量表征了6，6，6 ′，6 ′-d4二棕榈酰磷脂酰胆碱/短杆菌肽D双层膜中磷脂酰链的无序。在622 cm-1、646-649 cm-1和651-653 cm-1处的三种CD 2摇摆模式被分配给特定的构象异构体，用于确定肽存在下的紊乱，以及特定类别的构象异构体在总笨拙群体中的百分比。在44 ℃下，10：1和30：1脂质/肽混合物中的笨拙百分比分别为15%和17%。在34 ℃时，相应值分别为9.8%和2.6%。在44 ℃下，相对于多个曲折形式，（单一曲折弯曲+扭结）构象的百分比从30：1混合物中的78%急剧降低到10：1混合物中的15%。这些数据提供了跨膜肽紊乱磷脂凝胶相和有序液晶相的程度的第一个定量测量。
A quantitative infrared characterization of phospholipid acyl chain disordering in 6,6,6′,6′-d4 dipalmitoylphosphatidylcholine/ Gramicidin D bilayers has been made. Three CD2 rocking modes, at 622 cm-1, 646–649 cm-1, and 651–653 cm-1, assigned to particular conformers, were used to determine disorder in the presence of peptide, as well as percentages of particular classes of conformer within the total gauche population. At 44C, the gauche percentages in 10:1 and 30:1 lipid/peptide mixtures were 15% and 17%, respectively. At 34C, the corresponding values were 9.8% and 2.6%. The percentage of (single gauche bend + kink) conformers, relative to multiple gauche forms, decreases dramatically from 78% in the 30:1 mixture to 15% in the 10:1 mixture at 44C. These data provide the first quantitative measure of the extent to which a membrane-spanning peptide disorders phospholipid gel phases and orders liquid crystal phases.