First meiosis errors in immature oocytes generated by stimulated cycles
First meiosis errors in immature oocytes generated by stimulated cycles
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DOI：
10.1016/j.fertnstert.2006.02.083
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发表时间：
2006-09-01
影响因子：
6.7
通讯作者：
Gianaroli, Luca
中科院分区：
文献类型：
--
作者：
Magli, M. Cristina;Ferraretti, Anna P.;Gianaroli, Luca
目的：探讨第一极体（PB）活检检测到的染色体错误与卵母细胞核成熟度的关系。设计：回顾性研究。设置：生殖医学单位。患者：87个周期的PB活检检查非整倍体。指征为母亲年龄>= 38岁（49个周期）、重复IVF失败（22个周期）和其他（16个周期）。干预：分析体内和体外成熟卵母细胞中第一极体的染色体13、16、18、21和22。采用卵胞浆内单精子显微注射技术对正常卵母细胞进行人工授精。主要观察指标：分析卵母细胞的染色体状态、卵胞浆内单精子显微注射后的发育、妊娠和种植权。结果：体外成熟卵母细胞染色体异常的比例高于体内成熟卵母细胞（70% vs. 54%，P
Objective: To investigate chromosomal errors detected by first polar body (PB) biopsy in relation to the nuclear maturity of the oocytes.Design: Retrospective study.Setting: Reproductive medicine unit.Patient(s): Eighty-seven cycles were examined by PB biopsy for aneuploidy. Indications were maternal age >= 38 years (49 cycles), repeated IVF failures (22 cycles), and others (16 cycles).Intervention(s): First polar bodies were analyzed for the chromosomes 13, 16, 18, 21, and 22 in both in vivo and in vitro matured oocytes. Euploid oocytes were inseminated by intracytoplasmic sperm injection.Main Outcome Measure(s): Chromosomal status of the analysed oocytes, development after intracytoplasmic sperm injection, pregnancy, and implantation rights.Result(s): In in vitro matured oocytes, the proportion of chromosomal abnormalities was higher than in vivo matured oocytes (70% vs. 54%, P