NMR study on the protonation of imidazole ring of N-acetyl-L-histidine methylamide, a model for histidine residues exposed to aqueous solvent.
NMR study on the protonation of imidazole ring of N-acetyl-L-histidine methylamide, a model for histidine residues exposed to aqueous solvent.
复制标题
N-乙酰基-L-组氨酸甲酰胺咪唑环质子化的核磁共振研究，组氨酸残基暴露于水性溶剂的模型。
DOI：
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发表时间：
1978
期刊：
影响因子：
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通讯作者：
MiyazawaTatsuo
中科院分区：
文献类型：
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作者：
TanokuraMasaru;TasumiMitsuo;MiyazawaTatsuo
观察了N-乙酰基-L-组氨酸甲酰胺和L-组氨酸在水和重水中的质子NMR化学位移的pH滴定曲线。获得了每种离子的宏观pKa和固有化学位移。测定了咪唑环质子化的焓变和熵变。这些数据对于探讨蛋白质中组氨酸残基的微环境是有用的。
The pH titration curves of proton NMR chemical shifts were observed for N-acetyl-L-histidine methylamide and L-histidine in H2O and D2O. Macroscopic pKa and intrinsic chemical shifts for each ionic species were obtained. The enthalpy and entropy changes on protonation of the imidazole ring were determined. These data are useful for the discussions of microenvironments of histidine residues in proteins.