Functional analysis of FarA transcription factor in the regulation of the genes encoding lipolytic enzymes and hydrophobic surface binding protein for the degradation of biodegradable plastics in Aspergillus oryzae
Functional analysis of FarA transcription factor in the regulation of the genes encoding lipolytic enzymes and hydrophobic surface binding protein for the degradation of biodegradable plastics in Aspergillus oryzae
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DOI：
10.1016/j.jbiosc.2011.12.014
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发表时间：
2012-05-01
影响因子：
2.8
通讯作者：
Gomi, Katsuya
中科院分区：
文献类型：
--
作者：
Garrido, Sharon Marie;Kitamoto, Noriyuki;Gomi, Katsuya
FarA是Zn（II）（2）Cys（6）转录因子，其上调丝状真菌如构巢曲霉中在脂肪酸上生长所需的基因。FarA也与茄病镰刀菌的角质酶转录因子CTE 1 α高度相似，CTE 1 α与该植物病原体中的角质酶基因启动子结合。这项研究确定了FarA转录因子是否也在调节负责生产角质酶的基因中起作用，角质酶用于降解可生物降解的塑料，聚（丁二酸丁二醇酯-共-己二酸酯）（PBSA），在曲霉菌中。野生型和farA基因破坏菌株在具有乳化PBSA的基本琼脂培养基中生长，并且野生型在菌落周围显示出清晰的区域，而破坏剂则没有。Western Idol分析表明，角质酶蛋白CutL 1和疏水表面结合蛋白，如HsbA产生的野生型，但不是由破坏剂。此外，cutL 1，三酰甘油脂肪酶（tglA），和单-和二-酰基甘油脂肪酶（mdlB）基因以及hsbA基因的表达显着较低的破坏相比，野生型。这些结果表明，FarA转录因子将参与表达的cutL 1和hsbA基因所需的PBSA的降解，以及a;脂解基因，如mdlB和tglA的脂质水解。(C)2012年，生物技术学会，日本。All rights reserved.
FarA is a Zn(II)(2)Cys(6) transcription factor which upregulates genes required for growth on fatty acids in filamentous fungi like Aspergillus nidulans. FarA is also highly similar to the cutinase transcription factor CTE1 alpha of Fusarium solani which binds to the cutinase gene promoter in this plant pathogen. This study determines whether FarA transcriptional factor also works in the regulation of genes responsible for the production of cutinase for the degradation of a biodegradable plastic, poly(butylene succinate-co-adipate) (PBSA), in Aspergillus oryzae. The wild-type and the farA gene disruption strains were grown in minimal agar medium with emulsified PBSA, and the wild-type showed clear zone around the colonies while the disruptants did not. Western Idol analysis revealed that the cutinase protein CutL1 and a hydrophobic surface binding protein such as HsbA were produced by the wild-type but not by the disruptants. In addition, the expressions of cutL1, triacylglycerol lipase (tglA), and mono- and di-acylglycerol lipase (mdlB) genes as well as the hsbA gene were significantly lower in the disruptants compared to the wild-type. These results indicated that the FarA transcriptional factor would be implicated in the expression of cutL1 and hsbA genes that are required for the degradation of PBSA as well a; lipolytic genes such as mdlB and tglA for lipid hydrolysis. (C) 2012, The Society for Biotechnology, Japan. All rights reserved.