A novel effect of polymorphonuclear leukocytes in the facilitation of angiogenesis.
A novel effect of polymorphonuclear leukocytes in the facilitation of angiogenesis.
复制标题
多形核白细胞促进血管生成的新作用。
DOI：
10.1016/s0024-3205(00)00537-3
复制
发表时间：
2000
期刊：
影响因子：
6.1
通讯作者：
T. Yamamoto
中科院分区：
文献类型：
--
作者：
M. Yasuda;S. Shimizu;S. Tokuyama;T. Watanabe;Y. Kiuchi;T. Yamamoto
本研究的目的是检查是否多形核白细胞（PMNs）的粘附内皮细胞和/或活性氧（ROS）释放的PMNs是负责诱导血管生成。通过使用从牛胸主动脉（BAEC）获得的内皮细胞在I型胶原蛋白层上生长的管形成来评估血管生成。BAECs中加入PMNs可微弱地诱导血管生成。用中性粒细胞的选择性激活剂N-甲酰基-甲硫氨酰-亮氨酰-苯丙氨酸（FMLP）处理中性粒细胞，进一步增强了单独由中性粒细胞诱导的血管生成。用抗E-选择素和细胞间粘附分子-1（ICAM-1）的单克隆抗体研究了中性粒细胞（PMN）通过粘附分子与BAEC粘附在血管生成中的作用。这些抗体阻断PMN粘附BAECs和FMLP处理的PMN诱导的血管生成的增强。此外，FMLP处理的中性粒细胞的血管生成的增强被过氧化氢酶，H2 O2的清除酶阻断，但不是由超氧化物歧化酶（SOD）。这些结果表明，PMNs在体外诱导血管生成，并且由PMNs刺激血管生成的机制可能涉及PMNs粘附内皮细胞通过E-选择素和ICAM-1，和H2 O2，但不是超氧化物。因此，病理状态下激活的中性粒细胞不仅可以诱导组织损伤，而且还可以作为血管生成的调节因子。
The purpose of this study was to examine whether the adhesion of polymorphonuclear leukocytes (PMNs) to endothelial cells and/or reactive oxygen species (ROS) released from PMNs are responsible for inducing angiogenesis. Angiogenesis was assessed by tube formation using endothelial cells obtained from bovine thoracic aorta (BAECs) grown on a layer of collagen type I. Addition of PMNs to BAECs weakly induced angiogenesis. The angiogenesis induced by PMNs alone was further enhanced by treatment of the PMNs with N-formyl-methionyl-leucyl-phenylalanine (FMLP), a selective activator of PMN. The involvement of PMN adhesion to BAECs via adhesion molecules in angiogenesis was investigated by using monoclonal antibodies against E-selectin and intercellular adhesion molecule-1 (ICAM-1). These antibodies blocked both the PMN adhesion to BAECs and the enhancement of angiogenesis induced by FMLP-treated PMNs. Furthermore, the enhancement of angiogenesis by FMLP-treated PMNs was blocked by catalase, a scavenging enzyme of H2O2, but not by Superoxide dismutase (SOD). These results suggest that PMNs induce angiogenesis in vitro, and that the mechanism of stimulation of angiogenesis by PMNs may involve the adherence of PMNs to endothelial cells via E-selectin and ICAM-1, and H2O2, but not Superoxide. Thus, activated PMNs in pathological states may not only induce tissue injury, but may also function as regulators of angiogenesis.
影响因子：
4.4
作者：
A. Koch;A. Koch;L. Harlow;G. K. Haines;E. Amento;E. Unemori;Wei Lee Wong;R. Pope;N. Ferrara
通讯作者：
A. Koch;A. Koch;L. Harlow;G. K. Haines;E. Amento;E. Unemori;Wei Lee Wong;R. Pope;N. Ferrara
影响因子：
56.9
作者：
KOCH, AE;POLVERINI, PJ;STRIETER, RM
通讯作者：
STRIETER, RM
角膜新生血管的组织病理学。
DOI：
10.1001/archopht.1983.01040010472030
发表时间：
1983
期刊：
Archives of ophthalmology (Chicago, Ill. : 1960)
影响因子：
--
作者：
Schanzlin,DJ;Cyr,RJ;Friedlaender,MH
通讯作者：
Friedlaender,MH