Developing Cellular Systems In Vitro to Simulate Regeneration
Developing Cellular Systems In Vitro to Simulate Regeneration
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DOI：
10.1089/ten.tea.2014.0002
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发表时间：
2014-05-01
影响因子：
4.1
通讯作者：
Kirkpatrick, Charles James
中科院分区：
文献类型：
--
作者：
Kirkpatrick, Charles James
在过去的二十年中，体外细胞系统已经从主要的单细胞测试模型发展到研究用于替代的新生物材料的毒性作用，到用于再生的相关人类共培养系统，通常是祖细胞与新生物材料的组合。在理解细胞串扰及其对骨血管化的贡献方面已经取得了相当大的进展。未来的挑战包括使用已建立的生理，也就是说，非活化，干细胞壁龛作为一个平台，开发共培养模型，这将使真正的原位再生壁龛进行调查。缺氧和不断变化的炎症状态是需要纳入的因素。允许在水凝胶中掺入特定生物相关信号的聚合物合成的重大进展将有助于使这成为现实。
In the past two decades, cellular systems in vitro have progressed from predominantly monocellular testing models to study the toxic effects of new biomaterials for replacement to relevant human coculture systems for regeneration, often a combination of progenitor cells with novel biomaterials. Considerable progress has been made in understanding cellular cross talk and its contribution to the vascularization of bone. Future challenges include using the established physiological, that is, nonactivated, stem cell niches as a platform to develop coculture models, which will enable the true in situ regenerative niche to be investigated. Hypoxia and a changing inflammatory status are factors that need to be incorporated. Major advances in polymer synthesis permitting the incorporation of specific biologically relevant signals in hydrogels will help make this a reality.