Gut microbiota-mediated metabolic restructuring aggravates emotional deficits after anesthesia/surgery in rats with preoperative stress.
Gut microbiota-mediated metabolic restructuring aggravates emotional deficits after anesthesia/surgery in rats with preoperative stress.
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DOI：
10.3389/fimmu.2022.819289
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发表时间：
2022
影响因子：
7.3
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中科院分区：
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术前有应激反应的患者术后容易出现情绪障碍。然而，潜在的机制在很大程度上是未知的。在这里，我们基于16S rRNA基因测序和非靶向代谢组学技术，对麻醉/手术应激大鼠的微生物组成和特定代谢产物的变化进行了表征。因此，我们发现麻醉/手术加剧了大鼠在术前应激下的焦虑样和抑郁样行为。麻醉/手术后小胶质细胞被激活，IL-6和肿瘤坏死因子ɑ等促炎细胞因子表达上调。手术前应激暴露的大鼠术后肠道微生物区系和代谢产物组成与对照组不同。最后，在接受抗生素治疗的大鼠中，情绪障碍、代谢改变和神经炎症恢复正常。我们的发现提供了进一步的证据，表明肠道微生物区系的异常有助于在术前应激下的大鼠术后代谢重组、神经炎症和精神缺陷。
Patients with preoperative stress are prone to postoperative emotional deficits. However, the underlying mechanisms are largely unknown. Here, we characterize the changes of microbial composition and specific metabolites after anesthesia/surgery in rats with preoperative stress based on 16S rRNA gene sequencing and non-targeted metabolomics technique. Consequently, we found that anesthesia/surgery aggravated anxiety-like and depression-like behaviors in rats under preoperative stress. Microglia were activated and pro-inflammatory cytokines, including interleukin 6 (IL-6) and tumor necrosis factor ɑ (TNF-α) were upregulated after anesthesia/surgery. The postoperative gut microbiota and metabolite composition of rats exposed to preoperative stress differed from those of control rats. Lastly, emotional impairments, metabolic alterations, and neuroinflammation returned normal in antibiotics-treated rats. Our findings provide further evidence that abnormalities in the gut microbiota contribute to postoperative metabolic restructuring, neuroinflammation, and psychiatric deficits in rats under preoperative stress.