Global profiling of viral and cellular non-coding RNAs in Epstein Barr virus-induced lymphoblastoid cell lines and released exosome cargos
Global profiling of viral and cellular non-coding RNAs in Epstein Barr virus-induced lymphoblastoid cell lines and released exosome cargos
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DOI：
10.1016/j.canlet.2016.12.003
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发表时间：
2017-03-01
期刊：
影响因子：
9.7
通讯作者：
Conaldi, Pier Giulio
中科院分区：
文献类型：
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作者：
Gallo, Alessia;Vella, Serena;Conaldi, Pier Giulio
eb病毒转化的人淋巴母细胞样细胞系（LCL）是通过感染外周血单眼细胞获得的，已广泛用于人类遗传学、药物基因组学和免疫学研究。最近，外泌体的作用也被认为是EBV与宿主微环境之间的串扰的关键。由于LCL和LCL外泌体货物可能在维持持续感染中发挥作用，并且由于对LCL的非编码RNA知之甚少，因此我们工作的目的是将LCL中的这类RNA、细胞和病毒mirna以及细胞lncrna与来自相同供体的PBMC进行比较。在本研究中，我们首次证明了LCL表达的所有病毒mirna也都被包装在外泌体中，并且我们发现ebv-miR-BART3和ebv-miR-BHRF1-1这两个mirna在外泌体中更丰富，这表明了微囊状病毒microRNA转移。此外，IncRNA分析显示LCLs富含IncRNA H19和H19反义，并通过外泌体释放，提示其在调节肿瘤微环境中起主导作用。(C) 2016年作者。爱思唯尔爱尔兰有限公司出版。这是一篇基于CC BY-NC-ND许可（http://creativecommons.org/licenses/by-nc-nd/4.0/）的开放获取文章。
The human EBV-transformed lymphoblastoid cell line (LCL), obtained by infecting peripheral blood monocular cells with Epstein Barr Virus, has been extensively used for human genetic, pharmacogenomic, and immunologic studies. Recently, the role of exosomes has also been indicated as crucial in the crosstalk between EBV and the host microenvironment. Because the role that the LCL and LCL exosomal cargo might play in maintaining persistent infection, and since little is known regarding the non-coding RNAs of LCL, the aim of our work was the comprehensive characterization of this class of RNA, cellular and viral miRNAs, and cellular lncRNAs, in LCL compared with PBMC derived from the same donors. In this study, we have demonstrated, for the first time, that all the viral miRNAs expressed by LCL are also packaged in the exosomes, and we found that two miRNAs, ebv-miR-BART3 and ebv-miR-BHRF1-1, are more abundant in the exosomes, suggesting a microvescicular viral microRNA transfer. In addition, IncRNA profiling revealed that LCLs were enriched in IncRNA H19 and H19 antisense, and released these through exosomes, suggesting a leading role in the regulation of the tumor microenvironment. (C) 2016 The Authors. Published by Elsevier Ireland Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).