Histone deacetylase HDA6 enhances brassinosteroid signaling by inhibiting the BIN2 kinase
Histone deacetylase HDA6 enhances brassinosteroid signaling by inhibiting the BIN2 kinase
复制标题
组蛋白脱乙酰酶 HDA6 通过抑制 BIN2 激酶增强油菜素类固醇信号传导
DOI：
10.1073/pnas.1521363113
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发表时间：
2016-09-13
影响因子：
11.1
通讯作者：
Wang, Xuelu
中科院分区：
文献类型：
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作者：
Hao, Yuhan;Wang, Haijiao;Wang, Xuelu
油菜素内酯（brassinosteroids， BR）信号通路在植物发育中具有重要作用，但其关键调控因子BR- insensitive 2 （BR - insensitive 2）的调控机制尚不清楚。我们发现组蛋白去乙酰化酶HDA6可以与BIN2相互作用并使其去乙酰化以抑制其活性，这对BIN2的抑制和组蛋白去乙酰化酶在修饰非组蛋白中的功能提供了重要的见解。我们还发现这种抑制可能与植物的能量状态有关。糖原合成酶激酶3 （GSK3）样激酶在油菜素内酯（BR）、脱落酸和生长素信号传导中发挥重要作用，调控植物发育和胁迫反应的许多方面。拟南芥gsk3样激酶BR- insensitive 2 （BIN2）在BR信号通路中起关键负调控作用，但其调控机制尚不清楚。在这里，我们报道了组蛋白去乙酰化酶HDA6可以与BIN2相互作用并使其去乙酰化以抑制其激酶活性。hda6突变体在黑暗中表现出BR抑制表型，对BR生物合成抑制剂不太敏感。遗传分析表明HDA6通过BIN2调控BR信号。此外，我们发现BIN2的K189是一个乙酰化位点，可以被HDA6去乙酰化，从而影响BIN2的活性。葡萄糖可以影响植物中BIN2的乙酰化水平，表明其与细胞能量状态有关。这些发现为植物生长发育中gsk3样激酶的调控提供了重要的见解。
Significance The brassinosteroid (BR) signaling pathway has important functions in plant development, but the mechanisms that control its key regulator BR-INSENSITIVE 2 (BIN2) remain poorly understood. We found that the histone deacetylase HDA6 can interact with and deacetylate BIN2 to inhibit its activity, providing significant insights into the repression of BIN2 and the function of the histone deacetylase in modifying nonhistone proteins. We also found that this repression may be related to energy status in plants. Glycogen synthase kinase 3 (GSK3)-like kinases play important roles in brassinosteroid (BR), abscisic acid, and auxin signaling to regulate many aspects of plant development and stress responses. The Arabidopsis thaliana GSK3-like kinase BR-INSENSITIVE 2 (BIN2) acts as a key negative regulator in the BR signaling pathway, but the mechanisms regulating BIN2 function remain unclear. Here we report that the histone deacetylase HDA6 can interact with and deacetylate BIN2 to repress its kinase activity. The hda6 mutant showed a BR-repressed phenotype in the dark and was less sensitive to BR biosynthesis inhibitors. Genetic analysis indicated that HDA6 regulates BR signaling through BIN2. Furthermore, we identified K189 of BIN2 as an acetylated site, which can be deacetylated by HDA6 to influence BIN2 activity. Glucose can affect the acetylation level of BIN2 in plants, indicating a connection to cellular energy status. These findings provide significant insights into the regulation of GSK3-like kinases in plant growth and development.