Immobilization techniques for mono- and oligosaccharide microarrays
Immobilization techniques for mono- and oligosaccharide microarrays
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DOI：
10.1002/qsar.200640082
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发表时间：
2006-11-01
期刊：
影响因子：
--
通讯作者：
Guttman, Andras
中科院分区：
文献类型：
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作者：
Monzo, Alex;Guttman, Andras
单糖和寡糖微阵列在分析许多重要的生物过程中起着至关重要的作用，如DNA-糖相互作用，蛋白质-糖相互作用和糖-糖相互作用。基于DNA和蛋白质微阵列的早期成功，在过去几年中出现了用于糖固定化以制造碳水化合物微阵列的类似技术。将各种单糖和寡糖有效地固定到固体表面上对于成功制备碳水化合物微阵列是必不可少的。在本文中，我们回顾了不同的技术可用于共价和非共价固定的共轭和非共轭的简单和复杂的糖到调色板上的各种表面。我们还简要介绍了这一新兴领域的一些未来前景。
Mono- and oligosaccharide microarrays play an essential role in the analysis of many important biological processes, such as DNA-sugar interactions, protein-sugar interactions, and sugar-sugar interactions. Based on the earlier success of DNA and protein microarrays, similar technologies emerged in the past years for sugar immobilization in order to fabricate carbohydrate microarrays. Efficient immobilization of various mono- and oligosaccharides of interest onto solid Surfaces is indispensable for successful preparation of carbohydrate microarrays. In this paper, we review the different techniques available for covalent and noncovalent immobilization of conjugated and nonconjugated simple and complex sugars onto a palette of various surfaces. We also give a short overview on some of the future prospects of this emerging field.