ADH-induced recycling of fluid-phase marker from endosomes to the mucosal surface in toad bladder.
ADH-induced recycling of fluid-phase marker from endosomes to the mucosal surface in toad bladder.
复制标题
ADH 诱导流体相标记物从内体到蟾蜍膀胱粘膜表面的再循环。
DOI：
10.1152/ajpcell.1994.267.1.c32
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Wade,JB
中科院分区：
文献类型：
--
作者：
Coleman,RA;Wade,JB
在蟾蜍膀胱中，抗利尿激素（ADH）刺激的逆转导致顶膜水通道以及粘膜沐浴溶液中存在的任何液相标记物的内吞作用。我们用辣根过氧化物酶 (HRP) 装载囊泡，然后重新刺激膀胱并测量内吞 HRP 在粘膜浴中的再现。重新刺激的负载 HRP 的膀胱显示 HRP 释放在重新刺激后 15 分钟内急剧达到峰值。改变负荷和再刺激之间的间隔并没有显着改变 HRP 的释放。用毛喉素再刺激可产生与 ADH 相似的 HRP 释放值。 HRP 释放量与诱导的水渗透性大小相关。流体相标记物可以以激素依赖性方式从内体循环到顶端表面的证明表明，含有水通道的内吞膜能够响应激素再刺激而快速循环回到表面。此外，随着重复再刺激，标记物释放逐渐下降，总计<回收量的30%。该结果表明相对较大比例的回收标记到达非回收隔间。
In the toad urinary bladder, the reversal of antidiuretic hormone (ADH) stimulation results in the endocytosis of apical membrane water channels, along with any fluid-phase marker present in the mucosal bathing solution. We have loaded vesicles with horseradish peroxidase (HRP), then restimulated the bladders and measured the reappearance of endocytosed HRP in the mucosal bath. HRP-loaded bladders that were restimulated showed HRP release that peaked sharply within 15 min after restimulation. Varying the interval between loading and restimulation did not vary HRP release significantly. Restimulation with forskolin gave HRP release values similar to ADH. The amount of HRP released correlated with the magnitude of water permeability induced. The demonstration that fluid-phase markers can be recycled from endosomes to the apical surface in a hormone-dependent fashion indicates that endocytosed membrane, containing water channels, is able to rapidly recycle back to the surface in response to hormone restimulation. In addition, marker release declined progressively with repeated restimulation, totaling < 30% of the retrieved amount. This result indicates that a relatively large proportion of the retrieved marker reaches a nonrecycling compartment.