Osmium complexation of mismatched DNA: effect of the bases adjacent to mismatched 5-methylcytosine.
Osmium complexation of mismatched DNA: effect of the bases adjacent to mismatched 5-methylcytosine.
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DOI：
10.1021/bc800531z
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发表时间：
2009-02
影响因子：
4.7
通讯作者：
A. Nomura;Kazuki Tainaka;A. Okamoto
中科院分区：
文献类型：
--
作者：
A. Nomura;Kazuki Tainaka;A. Okamoto
错配DNA双链中锇配合物在5 - 甲基胞嘧啶处的形成效率是设计DNA甲基化序列特异性检测的关键。在完全匹配的双链中未观察到锇配合物形成，而错配双链中的配合位点和效率因形成错配碱基对的5 - 甲基胞嘧啶的5' - 相邻碱基类型而异。特别是当错配碱基对5'侧相邻的碱基为胸腺嘧啶时，观察到一种独特的“副反应”。然而，反应位点中错配碱基对的性质并不影响锇与甲基化DNA形成配合物的选择性。
The efficiency of osmium complex formation at 5-methylcytosine in mismatched DNA duplexes is a key point for the design of sequence-specific detection of DNA methylation. Osmium complexation was not observed in fully matched duplexes, whereas the complexation site and efficiency in mismatched duplexes changed depending on the type of 5'-neighboring base of the 5-methylcytosine forming a mismatched base pair. In particular, when the base adjacent to the 5' side of the mismatched base pair was thymine, a unique "side reaction" was observed. However, the nature of the mismatched base pairs in the reaction site did not influence the selectivity of osmium complex formation with methylated DNA.