High Performance Liquid Chromatography and Proteolytic Enzyme Characterization of Peptides in Tooth Pulp Extracts

High Performance Liquid Chromatography and Proteolytic Enzyme Characterization of Peptides in Tooth Pulp Extracts
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牙髓提取物中肽的高效液相色谱和蛋白水解酶表征

DOI:
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发表时间:
1982
期刊:
影响因子:
--
通讯作者:
D. Desiderio
D. Desiderio
中科院分区:
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文献类型:
--
作者:
H. E. May;F. Tanzer;G. Fridland;C. Wakelyn;D. Desiderio

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摘要:获得了牙髓提取物中所含肽的代谢谱。为了确定哪些高效液相色谱峰是由肽引起的,使用了一系列的蛋白水解酶(胰凝乳酶、胰蛋白酶和羧肽酶a)。用固定化酶处理提取物的结果表明,在这个反相系统中几乎所有的峰都是由于肽。目前的研究是一个更大的研究项目的必要组成部分,重点是量化脑和牙髓组织等生物提取物中的脑啡肽和内啡肽相关肽。
Abstract Metabolic profiles are obtained for peptides contained in tooth pulp extracts. To determine which high performance liquid chromatographic peaks are due to peptides, a series of proteolytic enzymes (chymotrypsin, trypsin, and carboxypeptidase A) are utilized. Results from treatment of extracts with immobilized enzymes demonstrate that virtually all peaks in this reverse phase system are due to peptides. This current study is a necessary component in a larger research program focusing on quantification of enkephalin-and endorphin-related peptides in biologic extracts including brain and tooth pulp tissue.
DOI: 10.1016/0003-2697(81)90162-7
发表时间: 1981
影响因子: 2.9
作者:
Mathews,WR;Rokach,J;Murphy,RC
通讯作者: Murphy,RC
DOI: 10.1002/bms.1200100604
发表时间: 1983
期刊: Biomedical mass spectrometry
影响因子: --
作者:
Desiderio,DM;Yamada,S
通讯作者: Yamada,S
DOI: 10.1016/0003-2697(82)90166-x
发表时间: 1982
影响因子: 2.9
作者:
Yamada,S;Desiderio,DM
通讯作者: Desiderio,DM