Intestinal immune system modulating polysaccharides in a Japanese herbal (Kampo) medicine, Juzen-Taiho-To
Intestinal immune system modulating polysaccharides in a Japanese herbal (Kampo) medicine, Juzen-Taiho-To
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DOI：
10.1078/094471102321616427
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发表时间：
2002-10-01
期刊：
影响因子：
7.9
通讯作者：
Yamada, H
中科院分区：
文献类型：
--
作者：
Kiyohara, H;Matsumoto, T;Yamada, H
商业上可以买到的日本草药(Kampo)处方的干提取物(TJ-48)在体外和体内都被发现可以增强Peyer‘s斑块细胞的功能(肠道免疫系统调节活性)。TJ-48经分离后，可透析组分(F-3)和多糖组分(F-5)表现出体外活性。小鼠灌胃给予F-5(150 mg/kg/d)对肠道免疫系统有调节作用。用内切多聚半乳糖醛酸酶降解F-5中果胶多糖的半乳糖醛酸半乳糖醛酸基时，消化产物显著提高了F-5的活性。经多聚半乳糖醛酸酶消化的F-5菌株的纯化表明，活性物质主要由耐酶或不能消化的多糖分子组成。F-5的凝胶过滤和阴离子交换层析分离得到12种多糖，其中7种多糖具有明显的肠道免疫调节活性。组分糖分析表明，一些活性多糖被分成含有阿拉伯半乳糖链或杂多糖链的果胶多糖。用β-D-葡糖-Yariv抗原进行单径向凝胶扩散分析表明，部分活性多糖含有阿拉伯-3，6-半乳糖部分，活性程度与与β-D-糖基-Yariv抗原的反应性之间的一元线性回归分析表明，该部分在活性表达中起重要作用。
A commercially available dried extract (TJ-48) of Japanese herbal (Kampo) prescription, Juzen-Taiho-To has been found to enhance functions of Peyer's patch cells (intestinal immune system modulating activity) in vitro and in vivo. When TJ-48 was fractionated, the dialyzable fraction (F-3) and the polysaccharide fraction (F-5) expressed the in vitro activity. Oral administration of F-5 (150 mg/kg/day) to mice showed the intestinal immune system modulating activity. When the galacturonan moiety of pectic polysaccharides in F-5 was degraded enzymatically by endo-polygalacturonase, the digestion products significantly increased the activity of F-5. Purification the polygalacturonase-digested F-5 indicated that the active substances were composed mainly of the enzyme-resistant or undigestable polysaccharide molecules. Gel filtrations and anion-exchange chromatographies of F-5 gave 12 kinds of polysaccharides, and among them, 7 polysaccharides had significant intestinal immune system modulating activity. Component sugar analysis suggests that some active polysaccharides are grouped into pectic polysaccharides containing arabinogalactan or heteroglycan chains. Single radial gel diffusion analysis using beta-D-glucosyl-Yariv antigen indicated that some of the active polysaccharides comprised arabino-3,6-galactan moiety, and this moiety was suggested to play an important role partly for expression of the activity by single linear regression analysis between degree of the activity and reactivity with beta-D-glyucosyl-Yariv antigen.